Page 30 - Application Notebook - Solution for Food Safety

Application No. C154
News

„ Quantitation
As one example, carbendazim was spiked into a matrix The limit on the number of MRM transations used to
at three different concentration levels. In Fig. 8, all MRM generate a product ion spectrum is dependent on the
transitions were detected even at the reporting level of chemical structure of the pesticide molecule. In the case
0.010mg/kg with a signal to noise for all fragment ion of carbendazim, several bonds could be broken using
transitions greater than 9. The response was linear for collision energies between 10-60V resulting in a product
all transitions throughout the calibration range (0.010- ion spectrum of 12 fragment ions. The product ion
0.200mg/kg) as shown Fig. 9. spectrum can then be used for library search and analyte
confirmation as shown in Fig. 10. For each calibration
level ranging from 0.010-0.200mg/kg the library
similarity score was greater than 99 confidently
confirming the target analyte. The advantage of this
technique is that library searchable product ion
spectrum data is used in target compound identification
without compromising sensitivity, accuracy and
robustness in quantitative data reporting.

Compound Name Carbendazim MRM spectrum Mode (Signal intensity 1e6) 160
Formula C9H9N3O2 Precursor-fragment ions Compound Name Carbendazim 132
CAS 10605-21-71 12 MRM’s Concentration 0.010 mg/kg
RT 4.42mins 1:192.10>159.95 CE: -34V Library Hit 100
2:192.10>132.10 CE: -32V 105
3:192.10>105.15 CE: -41V 2.5
4:192.10>65.10 CE: -48V 65
5:192.10>90.15 CE: -42V
90
6:192.10>92.15 CE: -36V 117
7:192.10>117.15 CE: -33V
8:192.10>78.15 CE: -55V 78
9:192.10>133.10 CE: -32V 0.0
10:192.10>51.10 CE: -60V 75.0 100.0 125.0 150.0 m/z
11:192.10>106.20 CE: -42V (Signal intensity 1e7)
12:192.10>78.90 CE: -50V
160
Compound Name Carbendazim 132
1.0 Concentration 0.050 mg/kg
Library Hit 100
105
By applying a range of collision energies to carbendazim
12 precursor-fragment ions are generated. MRM 192.10>159.95
was used in generating sensitive and robust quantitation whilst 0.5 65
the product ion spectrum using all 12 fragment ions was used in 90
confirming peak identification. 117
78
0.0
75.0 100.0 125.0 150.0 m/z
Peak Area (Signal intensity 1e7)
Compound Name Carbendazim 132 160
3.5 Compound Name Carbendazim
Linear regression curve No weighting 1.5
Regression R² = 0.9993576 Concentration 0.200 mg/kg
Library Hit 98 105
3.0
1.0
65
2.5 90
0.5
117
2.0 78
0.0
75.0 100.0 125.0 150.0 m/z
1.5

MRM Product ion spectrum data for carbendazim in 3
1.0
calibration levels (0.010-0.200mg/kg) spiked into a food matrix
was compared with an authentic library spectrum of
0.5 carbendazim. In all library searches the similarity score was
greater than 99 indicating a very high confidence in compound
verification and reporting.
0.0
0 0.05 0.10 0.15 0.20
Concentration (mg/kg)

Calibration curve for carbendazim using the optimized
quantitation ion transition (MRM 192.10>159.95). The response
was linear for all calibration and QC samples. All 12 fragment
ions were above a signal to noise ratio of 10 even at the
reporting level of 0.010mg/kg.