Page 7 - Application Notebook - Solution for Food Development

Application No.L467
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n Analysis of Orange Juice Table 2 Content of Each Sugar in Orange Juices
Fig. 3 and 4 show the chromatograms obtained from Content （g/L）
analysis of Orange Juice A and Orange Juice B, Orange juice A Orange juice B
respectively. Both Orange Juice A and B were diluted
with water to obtain 10-fold dilutions, respectively, and Sucrose 36 36
after filtering the solutions through a 0.2 µm membrane Glucose 25 28
filter, 10 µL each was injected. The analytical conditions Fructose 27 34
used were the same as those shown in Table 1. Sucrose,
glucose and fructose were detected in both types of
orange juice. Table 2 shows the content values for the
respective sugars detected in the juices.

n Analysis of Grape Juice
uRI
Fig. 4 shows a chromatogram obtained from analysis of
■ Peaks grape juice. The grape juice was diluted with water to
2. Sucrose
30 3. Glucose obtain a 10-fold dilution, and after filtering the solution
2 4. Fructose through a 0.2 µm membrane filter, 10 µL of the
prepared sample was injected. The analytical conditions
used were the same as those shown in Table 1. Glucose
20 4 and fructose were detected in the grape juice. Table 3
3 shows the content values of the detected sugars.

10
Table 3 Content of Each Sugar in Grape Juice
Content （g/L）
0 Glucose 50
Fructose 56
0.0 5.0 10.0 15.0 20.0 25.0 min

Fig. 3 Chromatogram of Orange Juice A (10 μL injected)

uRI uRI
50
■ Peaks ■ Peaks
2. Sucrose 3. Glucose
30 3. Glucose
2 4. Fructose 40 4. Fructose
3 4
4
20 3 30

20
10

10

0
0

0.0 5.0 10.0 15.0 20.0 25.0 min 0.0 5.0 10.0 15.0 20.0 25.0 min
Fig. 4 Chromatogram of Orange Juice B (10 μL injected) Fig. 5 Chromatogram of Grape Juice (10 μL injected)

First Edition: Aug. 2014

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