Page 5 - Application Notebook - Solution for Food Development

Application No.L459
News

mAU ■ Peaks
6
1. Thiamine Table 1 Analytical Conditions: Shim-pack MAqC-ODS Ⅰ
150 2. Niacin
3. Nicotinamide Column : Shim-pack MAqC-ODS Ⅰ
4. Pyridoxine (150 mm L. × 4.6 mm I.D., 5 µm)
7 5. Ca Pantothenate Mobile Phase : A) 10 mmol/L phosphate (sodium) buffer (pH 2.6)
100 3 4
2 6. Folic acid B) Acetonitrile
7. Riboflavin Time Program : B Conc. 1 % (0 min) → 1 % (2.5 min)
→ 50 % (10 min) → 1 % (10.01 min → 15 min)
50 1 8. Biotin Flowrate : 1.2 mL/min
5 8 Column Temp. : 40 ˚C
Detection : SPD-M20A at 210 nm
0 Injection Vol. : 10 µL
0 2 4 6 8 10
min

Fig. 2 Chromatogram of Standard Mixture of 8 Water-Soluble Vitamins (Shim-pack MAqC-ODS Ⅰ)

mAU
■ Peaks Table 2 Analytical Conditions: Shim-pack VP-ODS
1. Thiamine
150 Column : Shim-pack VP-ODS
3 2. Niacin (150 mm L. × 4.6 mm I.D., 5 µm)
2 3. Nicotinamide Mobile Phase : A) 100 mol/L phosphate (sodium) buffer (pH 2.1)
4 4. Pyridoxine Containing 0.8 mmol/L sodium 1-octanesulfonate
100
5. Ca Pantothenate B) Acetonitrile
6. Folic acid A/B = 10/1 (v/v)
7. Riboflavin Flowrate : 1.2 mL/min
50 8. Biotin Column Temp. : 40 ˚C
1 7 Detection : SPD-M20A at 210 nm
5 6 8 Injection Vol. : 10 µL
0
0 2 4 6 8 10 12 14
min
Fig. 3 Chromatogram of Standard Mixture of 8 Water-Soluble Vitamins (Shim-pack VP-ODS)

n Analysis of Multivitamin Tablet
Fig. 5 shows a chromatogram of a multivitamin tablet
that was analyzed using the Shim-pack MAqC-ODS Ⅰ
column. The analytical conditions were the same as
those shown in Table 1. Sample preparation was ■ Peaks
conducted according to the procedure outlined in Fig. 4. 1. Thiamine 2. Nicotinamide 3. Pyridoxine
4. Ca Pantothenate 5. Folic acid 6. Riboflavin
Tablet mAU
(dosage per day)
2 3 6
Pound in a
mortar
Add 20 mmol/L 500
aqueous sodium
hydroxide 10 mL
Mix
Add 10 mmol/L
phosphate 250
(sodium) buffer 90 mL 4
1
Mix
5
Filtrate 0
0 2 4 6 8 10
min
Inject to HPLC
Fig. 4 Sample Preparation Procedure Fig. 5 Chromatogram of Multivitamin Tablet

The Shim-pack MAqC-ODS Ⅰ was developed jointly with Eisai Co., Ltd.
First Edition: Aug. 2014

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