Page 40 - Application Notebook - Solution for Food Development

For LabSolutions LCMS
LC/MS/MS Method Package for D/L Amino Acids

All D/L Amino Acids Can Be Quantified by Column Switching

The physicochemical properties of Glutamine and Lysine, Isoleucine and allo-Isoleucine, Threonine and allo-Threonine are extremely similar.
They have virtually the same MS/MS fragmentation patterns, and share many of their MRM transitions. Chromatographic separation is
therefore required to analyze them individually by LC-MS/MS. Even if there is coelution on the CR-I(+) column, confirmation can be made by
automated switching to a secondary CR-I(-) column.

CROWNPAK CR-I(+) CROWNPAK CR-I(-)

132>86 L-Ile + L-allo-Ile 132>86
D-Ile + D-allo-Ile
L-Leu
D-Leu
D-allo-Ile
Analysis of D/L-Isoleucine, L-allo-Ile
D/L-allo-Isoleucine, L-Ile L-Leu D-Ile
and D/L-Leucine D-Leu

1.0 2.0 min 1.0 2.0 min

120>74 120>74
D-Thr + D-allo-Thr L-Thr + L-allo-Thr

D-allo-Thr
L-allo-Thr
Analysis of D/L-Threonine,
and D/L-allo-Threonine L-Thr D-Thr

0.5 1.0 1.5 min 0.5 1.0 1.5 min

List of Registered Amino Acids

D/L-Alanine D/L-Cysteine D/L-Histidine D/L-Lysine D/L-Serine D/L-Tyrosine
D/L-Arginine D/L-Glutamine D/L-Isoleucine D/L-Methionine D/L-Threonine D/L-Valine
D/L-Asparagine D/L-Glutamic acid D/L-allo-Isoleucine D/L-Phenylalanine D/L-allo-Threonine
D/L-Aspartic acid Glycine D/L-Leucine DL-Proline D/L-Tryptophane

Precautions
1. DL-Proline is a secondary amine, so it cannot be separated with these analysis conditions.
2. LabSolutions LCMS Ver. 5.86 or later is required.
CROWNPAK CR-I(+) and CR-I(-) are products of Daicel Corporation.

© Shimadzu Corporation, 2017
First Edition: March 2017, Printed in Japan 3655-12611-20AIT