Page 39 - Application Notebook - Solution for Food Development

C146-E336

For LabSolutions LCMS
LC/MS/MS Method Package

for D/L Amino Acids

LCMS-8060

Most important amino acids exist as stereoisomers. D- and L- forms of mirror image isomers, or enantiomers, are named according to their
activity on polarized light. By using CROWNPAK CR-I(+) and CR-I(-) columns with chiral stationary phases, the D- and L-forms of amino acids
can be analyzed separately. With CR-I(+) elution order is from D- to L-, and with CR-I(-) the elution order is reversed.

In Just Ten Minutes, Chiral Amino Acids Can Be Analyzed Simultaneously
With conventional chiral amino acid analysis, it is necessary to perform derivatization or use very long run times. With this method
package, derivatization is not necessary, and high-sensitivity analysis can be performed in a short period of time, bringing efficiency to
the chiral separations laboratory.
HPLC Conditions
CROWNPAK CR-I(+)
Column : CROWNPAK CR-I(+)/(-)
(3 mmI.D. × 150 mmL., 5 µm)
Mobile Phase : Acetonitrile/Ethanol/Water/TFA = 80/15/5/0.5
Flowrate : 0.6 mL/min
Injection Volume : 1 µL
Column Temp. : 25 °C

0.0 1.0 2.0 3.0 4.0 5.0 6.0 min
MS Conditions
CROWNPAK CR-I(-)
Nebulizer Gas Flowrate : 3.0 L/min
Drying Gas Flowrate : 15.0 L/min
Heating Gas Flowrate : 5.0 L/min
Interface Temp. : 250 °C
DL Temp. : 250 °C
Heat Block Temp : 300 °C
0.0 1.0 2.0 3.0 4.0 5.0 6.0 min