Page 54 - Application Notebook - PFAS Analysis

No. SSI-HPLC-037

Table 3: % recovery of target PFAS Quantitative analysis of fish samples
Three fish samples with unknown PFAS
Compound % recovery
PFBS 98.7 concentrations were then evaluated with this
PFHxA 105.9 method. The samples were wild caught Walleye, wild
HFPO-DA 97.4 caught Large Mouth Bass, and farm raised Trout.
PFHxS 102.7 Figure 3 shows the LC-MS/MS chromatogram of an
PFHpA 100.5 extracted sample from each fish’s type. Table 6
ADONA 100.7 shows the concentration of PFAS determined in each
PFOA 104.2 type of fish. The wild caught Walleye and Large
PFNA 101.9
PFOS 98.1 Mouth Bass were found to contain the largest
9Cl-PF3ONS 100.5 amounts of PFOS, PFDA, and PFUnA. No PFAS
PFDA 99.9 compounds were detected above the LOQ in the
N-MeFOSAA 102.2 farm raised Trout sample.
N-EtFOSAA 97.6
PFUnA 94.6
11Cl-PF3OUds 102.2
PFDoA 96.3
PFTriA 99.8
PFTreA 97.2

Table 4: Linearity of PFAS compounds spiked onto fish tissue

Lowest Calibration Standard (LOQ) Highest Calibration Standard Linearity (R )
2
ng/g spiked on fish ng/g spiked on fish
PFBS 0.5 100 0.9999
PFHxA 0.5 100 0.9995
HFPO-DA 1 100 0.9997
PFHpA 1 100 0.9996
PFHxS 0.5 100 0.9999
ADONA 0.5 100 0.9997
PFOA 0.5 100 0.9997
PFNA 0.5 100 0.9997
PFOS 2 100 0.9999
9Cl-PF3ONS 1 100 0.9995
PFDA 0.5 100 0.9998
N-MeFOSAA 2 100 0.9994
N-ETFOSAA 1 100 0.9999
PFUnA 1 100 0.9997
11Cl-PF3OUdS 0.5 100 0.9999
PFDoA 1 100 0.9996
PFTriA 2 100 0.9997
PFTreA 1 100 0.9995

Table 5: Reproducibility of PFAS SFE extractions (n=3)

%RSD
100 ng/g 20 ng/g 2 ng/g
PFBS 2.3 7.9 21.7
PFHxA 4.9 4.1 15.6
HFPO-DA 3.9 4.4 9.9
PFHxS 4.2 4.4 19.9
PFHpA 2.6 4.9 2.4
ADONA 3.9 3.2 13.2
PFOA 2.9 3.1 13.1
PFNA 3.5 3.6 18.1
PFOS 4.1 3.9 22.1
9Cl-PF3ONS 2.5 1.3 3.6
PFDA 1.6 7.4 20.9
N-MeFOSAA 9.5 9.6 44.7
N-EtFOSAA 8.4 6.2 10.7
PFUnA 2.3 2.8 18.4
11Cl-PF3OUds 4.1 4.9 7.8
PFDoA 4.7 5.8 15.9
PFTriA 4.4 11.6 26.8
PFTreA 2.3 3.6 11.5