Page 50 - Application Notebook - PFAS Analysis
P. 50
No. SSI-LCMS-121
Day 1 Day 2 Day 3 Day 4 Day 5 AVE
160
140
120
% Recovery 80
100
60
40
20
0
PFBA PFMPA PFPeA PFBS PFMBA PFEESA NFDHA 4-2 FTS PFHxA PFPeS HFPO-DA PFHpA PFHxS ADONA 6-2 FTS PFOA PFHpS PFNA PFOS 9Cl-PF3ONS 8-2 FTS PFDA PFUnA 11Cl-PF3OUdS PFDoA
Figure 6: %recovery (individual injections from five consecutive days and average) at MRL concentration.
RW TW
140
120
100
% Recovery 80
60
40
20
0
PFBA PFMPA PFPeA PFBS PFMBA PFEESA NFDHA 4-2 FTS PFHxA PFPeS HFPO-DA PFHpA PFHxS ADONA 6-2 FTS PFOA PFHpS PFNA PFOS 9Cl-PF3ONS 8-2 FTS PFDA PFUnA 11Cl-PF3OUdS PFDoA
Figure 7: Precision and accuracy results.
■ Conclusions
This study showed good chromatographic separation This data was generated as part of the EPA method
for all compounds listed in the method using the 533 second laboratory validation organized by EPA.
delay and analytical columns recommended by EPA. Shimadzu participated in this validation, as
Recoveries for most target compounds and precision acknowledged in the final method.
and accuracy data for all target analytes in reagent
water and tap water were within EPA requirements
of 70 -130%, with %RSD below 20% for all method
analytes.
■ References
1. EPA method 533: Determination of Per- and Polyfluoroalkyl Substances in Drinking Water by Isotope
Dilution anion exchange Solid Phase Extraction and Liquid Chromatography/Tandem Mass
Spectrometry(U.S. Environmental Protection Agency, Washington, D.C., December 2019).
2. Shimadzu Application News No. C184, “Analysis of PFAS Specified in EPA Method 537 and Beyond Using
Shimadzu UFMS”, 2019.
