Page 29 - Application Notebook - PFAS Analysis

SSI-LCMS-102

Precision and Accuracy Study
A precision and accuracy study was performed to assess Table 6 shows the results for the precision and accuracy
the long-term performance of the instrument. Eight study.
replicates of a 40 ppt and 80 ppt sample concentration
were injected. The percent recovery for all compounds was
within ±15% for both concentrations. All QC requirements
for EPA Method 537.1 were met. These requirements
include %RSD of less than 20% and peak asymmetry
factors for PFBS and PFHxA (first two compounds eluting in
the method) between 0.8 and 1.5 (calculated for a mid-
level calibration standard).

Table 6: Precision and Accuracy Study Results at 40 ppt sample concentration
Fortified Average Percent Fortified Average Percent
Compound %RSD %RSD
Concentration Concentration Recovery Concentration Concentration Recovery
PFBS 40 43.7 109 3.9 80 87.7 110 5.1
PFHxA 40 43.5 109 4.2 80 85.9 107 5.0
HFPO-DA 40 39.6 99 4.8 80 86.5 108 5.0
PFHpA 40 44.7 112 4.7 80 88.6 111 6.2
PFHxS 40 43.9 110 18.5 80 87.0 109 4.9
ADONA 40 40.6 101 4.2 80 88.6 111 7.4
PFOA 40 42.4 106 4.7 80 84.6 106 5.7
PFNA 40 44.2 110 5.5 80 88.3 110 5.4
PFOS 40 44.6 111 5.5 80 90.0 113 8.3
9Cl-PF3ONS 40 41.1 103 5.0 80 82.7 103 7.4
PFDA 40 42.5 106 3.9 80 84.2 105 5.6
N-MeFOSAA 40 44.1 110 5.7 80 87.0 109 7.7
N-EtFOSAA 40 42.7 107 6.4 80 84.2 105 7.5
PFUnA 40 43.0 108 5.2 80 85.7 107 5.8
11Cl-PF3OUdS 40 41.7 104 4.9 80 82.8 103 10.2
PFDoA 40 43.6 109 4.9 80 85.5 107 5.4
PFTriA 40 42.3 106 5.9 80 85.0 106 5.1
PFTreA 40 42.7 107 3.8 80 84.8 106 5.9