Page 16 - Application Notebook - PFAS Analysis

Application No. C184
News

PFAS (e.g. PFOA and PFOS), there are other classes  Experimental
gaining attention due to their increasing use,
occurrence and persistence in the environment. In this PFAS and Preparation of Calibration Standards
study, apart from the compounds listed in EPA Method Table 1 lists the 27 PFAS compounds (21 target
537, the scope of PFAS has been expanded to include compounds, 3 internal standards (IS) and 3
seven additional compounds such as fluorotelomeric surrogates) used in this study. All PFAS standards
alcohols (precursor of PFOA). were purchased from Wellington Laboratories (Guelph,
This application news describes and demonstrates the Ontario). A series of 10 calibration standards at
use and performance of Shimadzu UFMS™ for the concentrations of 1.25, 2.50, 5.00, 10.0, 15.0, 20.0,
analysis of 21 PFAS (including all 14 stated in EPA 25.0, 37.5, 50.0 and 100 ng/mL were prepared by
Method 537) in drinking water. Shimadzu triple dilution with 96:4% (vol/vol) methanol:water. These
quadrupole mass spectrometers, LCMS-8045 and concentrations were 250 times higher than the target
LCMS-8050, were used in this study. Possessing an concentration range in consideration of analyte
ultra-fast acquisition rate of 555 MRM/sec and a high enrichment over the course of sample preparation,
polarity switching speed (5 msec), these UFMS™ such that a 1.25 ng/mL calibration standard was
instruments achieve rapid, reliable and highly-sensitive equivalent to 5 ng/L of field sample.
quantitation of PFAS in drinking water.
Table 1. List of PFAS (target compounds, internal standards and surrogates)

PFAS Listed
Molecular Molecular IS, Surrogates and its
PFAS Compounds Abbreviation CAS Weight Formula Abbreviation in EPA
Method 537
PERFLUOROALKYLCARBOXYLIC ACIDS
M2PFHxA (Surr.)
Perfluorohexanoic acid PFHxA 307-24-4 314.06 C 6 F 11 O 2 H ( C 2 C 4 F 11 O 2 H) 
12
13
Perfluoroheptanoic acid PFHpA 375-85-9 364.06 C 7 F 13 O 2 H - 
M2PFOA (IS)
Perfluorooctanoic acid PFOA 335-67-1 414.07 C 8 F 15 O 2 H 
( C 2 C 6 F 15 O 2 H)
13
12
Perfluorononanoic acid PFNA 375-95-1 464.08 C 9 F 17 O 2 H - 
M2PFDA (Surr.)
Perfluorodecanoic acid PFDA 335-76-2 514.09 C 10 F 19 O 2 H 
( C 2 C 8 F 19 O 2 H)
12
13
Perfluoroundecanoic acid PFUnA 2058-94-8 564.09 C 11 F 21 O 2 H - 
Perfluorododecanoic acid PFDoA 307-55-1 614.10 C 12 F 23 O 2 H - 
Perfluorotridecanoic acid PFTriA 72629-94-8 664.11 C 13 F 25 O 2 H - 
Perfluorotetradecanoic acid PFTreA 376-06-7 714.12 C 14 F 27 O 2 H - 
PERFLUOROALKYLSULFONATES
Perfluorobutyl sulfonate PFBS 375-73-5 300.10 C 4 F 9 SO 3 H - 

Perfluoropentane sulfonate PFPeS 2706-91-4 350.11 C 5 F 11 SO 3 H - Additional
Perfluorohexyl sulfonate PFHxS 355-46-4 400.11 C 6 F 13 SO 3 H - 
Perfluorohexyl sulfonate PFHpS 375-92-8 450.12 C 7 F 15 SO 3 H - Additional
M4PFOS (IS)
Perfluoroheptane sulfonate PFOS 1763-23-1 500.13 C 8 F 17 SO 3 H 13 12 
( C 4 C 4 F 17 SO 3 Na)
Perfluorooctyl sulfonate PFNS 68259-12-1 550.14 C 9 F 19 SO 3 H - Additional
Perfluorononane sulfonate PFDS 335-77-3 600.14 C 10 F 21 SO 3 H - Additional

FLUORINATED TELOMER SULFONATES
1H, 1H, 2H, 2H-perfluorohexane sulfonic acid 4:2 FTS 757124-72-4 328.15 C 6 H 5 F 9 SO 3 - Additional

1H, 1H, 2H, 2H-perfluorooctane sulfonic acid 6:2 FTS 27619-97-2 428.17 C 8 H 5 F 13 SO 3 - Additional
1H, 1H, 2H, 2H-perfluorodecane sulfonic acid 8:2 FTS 39108-34-4 528.18 C 10 H 5 F 17 SO 3 - Additional
PERFLUOROOCTANESULFONAMIDE AND PERFLUOROOCTANESULFONAMIDOACETIC ACIDS
d3-NMeFOSAA (IS)
2-(N-Methylperfluorooctanesulfonamido) acetic acid N-MeFOSAA 2355-31-9 571.21 C 11 H 6 F 17 NSO 4 2 
(C 11 H 3 H 3 F 17 NSO 4 )
d5-NEtFOSAA (Surr.)
2-(N-Ethylperfluorooctanesulfonamido) acetic acid N-EtFOSAA 2991-50-6 585.24 C 12 H 8 F 17 NSO 4 2 
(C 12 H 5 H 3 F 17 NSO 4 )