Page 68 - LC-SFC_Pharma_Brochure

3. Summary of i-DReC Settings 2. A mark indicating whether i-DReC has been applied, the wave-
length used for correction, the retention time of the spectrum
i-DReC parameters are set as part of the data processing param-
used for sensitivity correction, and the calculated sensitivity
eters for the photodiode array detector and can be applied in
correction factor can be shown in the peak table and com-
data processing methods for routine analysis without requiring
pound table.
post-run operations. The following is a summary of parameters
and their descriptions. Item Description
C = peaks to which i-DReC was applied
Mark
1. i-DReC parameters are set in the multi chromatogram table. E1-E4 = error in i-DReC calculation
For peaks to which i-DReC was applied,
DRE wavelength
this is the wavelength used for correction.
For peaks to which i-DReC was applied,
DRE factor this is the absorption ratio used for
sensitivity correction.
For peaks to which i-DReC was applied,
DRE time
this is the retention time of the spectrum
used for sensitivity correction.
i-DReC requires that spectrum similarity is maintained across the
peak. When peak separation is insufficient, i-DReC may not be
able to be applied.
3. The peak area and height corrected by i-DReC can be used in
normal quantitation processes. The simple implementation allows
the seamless use of i-DReC for routine analysis.

Parameter Description
Dynamic range extension Select whether i-DReC is applied or not. 4. Conclusion
When intensity at peak top is over the
Threshold threshold, i-DReC is applied to the peak i-DReC's ability to calculate corrected peak area and height for
for correction.
high concentration samples is made possible by the improved
Wavelength for correction Select whether wavelength for correction
(manual/auto) will be set manually or automatically. performance of the SPD-M30A photodiode array detector and
When "Manual" is selected, set the the excellent reproducibility of the Nexera X2 system. The key fea-
Ch# channel number of the chromatogram tures of i-DReC can be summarized as follows.
that will be used.
Extension of the linear dynamic range using spectral similarity.
When "Auto" is selected, set the target
Intensity for
intensity for determining an appropriate
correction wavelength Simultaneous quantitation of both low and high concentration
correction wavelength.
compounds in a single injection.
When "Auto" is selected, set the direction
to search for an appropriate correction Requires the use of only one PDA detector.
Direction (+/-)
wavelength. (+ = longer wavelength,
– = shorter wavelength) Standard samples are not necessary for correction.
Set intensity for extraction of sensitivity
Intensity to extract Simple method settings allow the use of i-DReC in routine anaysis.
correction spectrum.
Select whether background compensation i-DReC can be applied to samples containing a wide range of
Background compensation is used or not for sensitivity correction
spectrum. compound concentrations. The use of i-DReC improves the effi-
ciency of sample pretreatment processes and laboratory produc-
The concentration range over which i-DReC effectively extends the tivity.
linear dynamic range is dependent upon the shape of the spectrum
of target peaks. The lower the slope of the spectrum around the
correction wavelength, the more reliable the correction.

First Edition: March, 2013

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