Page 36 - Application Handbook - Liquid Chromatography

Application No.L477
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n Repeatability of Peak Area Values
Tables 2 and 3 show the peak area %RSD values V7
obtained for each carbon number based on 6 repeat n 1FBLT
measurements of standard solutions of three anionic $
$
surfactants, each at a concentration of 1 mg/L and $
5 mg/L. For all samples, the area %RSD was 0.6 % or $
less for both the 1 mg/L and 5 mg/L concentrations. $

Table 2 Reproducibility of Peak Area (%RSD) from Repeat Injections
(Using Column 1)
Upper: Standard Solution Containing 1 mg/L of Each Substance
Lower: Standard Solution Containing 5 mg/L of Each Substance
5BQ 8BUFS 4QJLFE 4UBOEBSE
Each at 1 mg/L (n=6)

C10 C11 C12 C13 C14 5BQ 8BUFS
%RSD 0.36 0.36 0.47 0.39 0.43
Each at 5 mg/L (n=6)
C10 C11 C12 C13 C14 NJO
%RSD 0.31 0.31 0.31 0.26 0.30
Fig. 4 Chromatograms of Tap Water (20 µL Inj.) (Using Column 1)
Upper: Water, 0.04 mg/L each, total 0.2 mg/L spiked
Table 3 Reproducibility of Peak Area (%RSD) from Repeat Injections Lower: Water
(Using Column 2)
Upper: Standard Solution Containing 1 mg/L of Each Substance
Lower: Standard Solution Containing 5 mg/L of Each Substance
Each at 1 mg/L (n=6)
C10 C11 C12 C13 C14 V7
%RSD 0.39 0.41 0.38 0.60 0.34
n 1FBLT
Each at 5 mg/L (n=6) $
C10 C11 C12 C13 C14 $
$
%RSD 0.18 0.16 0.16 0.14 0.15 $

$
n Analysis of Tap Water
The RF-20Axs high-sensitivity fluorescence detector
features a temperature-controlled cell with a cooling
function and is ideal for the direct injection of neat test
water into the HPLC. The cell temperature control
minimizes any fluorescence quenching at elevated or
changing ambient temperature conditions. 5BQ 8BUFS 4QJLFE 4UBOEBSE
Figs. 4 and 5 show examples of tap water analysis in
which the undiluted samples were injected directly into 5BQ 8BUFS
the HPLC. In each pair of analysis results, the sample
associated with the upper chromatogram consisted of NJO
tap water spiked with the five anionic surfactants
subject to water quality standard limits (each 0.04 mg/L, Fig. 5 Chromatograms of Tap Water (20 µL Inj.) (Using Column 2)
total of 0.2 mg/L) . The lower trace is a water sample Upper: Water, 0.04 mg/L each, total 0.2 mg/L spiked
1)
with no surfactants added. The analytical conditions are Lower: Water
the same as those shown in Table 1.
In the Fig. 4 results from the column which resolves
branched chains, a signal-to-noise ratio S/N=6 was
obtained for the peak with the lowest intensity (third
isomer of C14).

[References]
1) Ordinance No. 101 of Japan's Ministry of Health, Labour and Welfare, May 30, 2003, (Partially revised by Ordinance No. 11 of Ministry of Health,
Labour and Welfare, January 28, 2011)
2) Ordinance No. 261 of Japan's Ministry of Health, Labour and Welfare, July 22, 2003, (Partially revised by Ordinance No. 290 of Ministry of Health,
Labour and Welfare, March 30, 2012)

First Edition: Nov. 2014

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