Page 5 - Pharmaceutical M5 Biopharma

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Sponsored Feature  5

Intact Proteins Robust and reliable separation and measurement

CQAs observable at intact protein level Analytical method

Aggregation, dimerization Size-exclusion chromatography (SEC)
Deamidation, pyroglutamination Charge-variant analysis (IEX)
Disulfide bond scrambling
Major glycoforms Molecular weight measurement (LC-MS)
Drug conjugation Hydrophobic interaction chromatography
(HIC)

Accurate Mass Analysis

Purified Mass
Protein Spectrometry

Accurate mass measurement helps to determine whether the
correct protein sequence has been expressed with the expected
post-translational modifications (PTMs). It also provides relative
abundance of different proteins or PTMs present in the same
sample. A high resolution and high sensitivity mass spectrometer
will facilitate this analysis.
Shimadzu offers the best solution for a routine high-resolution
accurate-mass analysis with the LCMS-9030, quadrupole time-
of-flight mass spectrometer (Q-TOF LC-MS). It maintains its
mass accuracy for days without requiring re-calibration or using
internal standard.

www.shimadzu.com