Page 4 - Pharmaceutical M5 Biopharma

4  Sponsored Feature

Intact Proteins Robust and reliable separation and measurement

Due to the complexity of the cellular
process controlling the synthesis and UV Analytical Conditions
translocation of macromolecules, the Sample : Anti-Apolipoprotein
efficiency at which host cells secrete the A-I Mouse mAb (CALBIOCHEM)
target protein into culture medium is Conc. : 60 μg/mL
largely dependent on individual cell line. Slit width : 1.0 nm
Thus, at an early stage of biopharmaceutical Scan range : 220 to 350 nm
development, screening is performed for Scan speed : medium
selecting a cell line that is most suited as Sampling interval : 0.5 nm
the vehicle for the product manufacturing.
Here, analysis of intact proteins are
performed to characterize few of the most
important quality attributes for safety and
efficacy. These attributes continue to be
monitored throughout the developmental
workflow, and thus require robust systems
and data integrity compliance.

Titer & Protein Quantification

How much protein is produced under
a given condition is a critical factor
affecting manufacturing productivity RF Analytical Conditions
and ultimately the long-term profitability Sample : Anti-Apolipoprotein A-I
of the final product. Shimadzu has Mouse mAb (CALBIOCHEM)
developed reliable spectroscopic and Conc. : 60 μg/mL
chromatographic solutions for protein Spectrum mode : emission
concentration measurements. Excitation : 280 nm
wavelength
Scan range : 280 nm to 500 nm
Band width : 3 nm (excitation/emission)
Technical report: Scan speed : slow
Data Integrity Compliance: Sampling : 1 nm
An Innovative Solution for interval
Molecular Spectroscopy Sensitivity : high