Page 69 - Pharmaceutical Solution for Pharma Analysis

Analysis of styrene leached from polystyrene cups
using GCMS coupled with Headspace (HS) sampler

(x1,000,000) Area
m/z : 104.00 1250000
2
2.00 R = 0.9996
1.75 50 ppb
1.50 1000000
20 ppb
1.25
10 ppb 750000
1.00
5 ppb
0.75 2.5 ppb 500000
0.50 1 ppb
0.25 250000
0.00
0
2.2 2.3 2.4 2.5 2.6 min 0 10 20 30 40 Conc.
Figure 6. Overlay of SIM chromatograms for m/z 104 at linearity levels Figure 7. Calibration curve for Styrene

Quantitation of styrene in polystyrene cup sample

Analysis of leachable styrene from polystyrene cups was styrene solutions in polystyrene cups. Figure 8 shows
done as per method described earlier. Recovery studies overlay SIM chromatogram of spiked and unspiked
were carried out by spiking 2.5, 10 and 50 ppb of standard samples. Table 3 shows the summary of results.

(x100,000)
m/z : 104.00

7.5

5.0 Spiked

2.5 Unspiked

0.0
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 min
Figure 8. Overlay SIM chromatograms of spiked and unspiked samples

Table 3. Summary of results for sample analysis

Observed Spiked
Sr. No. Sample Name Parameter Concentration Concentration % Recovery
in ppb in ppb
1 Unspiked sample Precision 9.8 NA NA
12.0 2.5 88.0
2 Spiked polystyrene cups Recovery 18.5 10 87.0
55.9 50 92.2

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