Page 66 - Pharmaceutical Solution for Pharma Analysis
P. 66
Analysis of styrene leached from polystyrene cups
using GCMS coupled with Headspace (HS) sampler
Table 1. Method validation parameters
Parameter Concentration (ppb)
Linearity 1, 2.5, 5, 10, 20, 50
Accuracy / Recovery 2.5, 10, 50
Precision at LOQ level 1
Reproducibility 50
HS-GCMS Analytical Conditions
Figure 3 shows the analytical instrument, HS-20 coupled with GCMS-QP2010 Ultra on which samples were analyzed with
following instrument parameter.
Figure 3. HS-20 coupled with GCMS-QP2010 Ultra by Shimadzu
HS-GCMS analytical parameters
Headspace parameters
• Sampling Mode : Loop
• Oven Temp. : 80.0 ºC
• Sample Line Temp. : 130.0 ºC
• Transfer Line Temp. : 140.0 ºC
• Equilibrating Time : 20.00 min
• Pressurizing Time : 0.50 min
• Pressure Equilib. Time : 0.10 min
• Load Time : 0.50 min
• Load Equilib. Time : 0.10 min
• Injection Time : 1.00 min
• Needle Flush Time : 10.00 min
• GC Cycle Time : 23.00 min
3
