Page 67 - Pharmaceutical Solution for Pharma Analysis

Analysis of styrene leached from polystyrene cups
using GCMS coupled with Headspace (HS) sampler

Chromatographic parameters
• Column : Rxi-5Sil MS (30 m L x 0.25 mm I.D., 0.25 m)
• Injection Mode : Split
• Split Ratio : 10.0
• Carrier Gas : Helium
• Flow Control Mode : Linear Velocity
• Linear Velocity : 36.3 cm/sec
• Pressure : 53.5 kPa
• Column Flow : 1.00 mL/min
• Total Flow : 14.0 mL/min
• Total Program Time : 12.42 min
• Column Oven Temp. : Rate (ºC /min) Temperature (ºC) Hold time (min)
50.0 0.00
40.00 200.0 1.00
30.00 280.0 5.00

Mass Spectrometry parameters

• Ion Source Temp. : 200 ºC
• Interface Temp. : 230 ºC
• Ionization Mode : EI
• Event Time : 0.20 sec
• Mode : SIM
• m/z : 104,103 and 78
• Start Time : 1.00 min
• End Time : 5.00 min

Results

Fragmentation of styrene
Mass spectrum of styrene is shown in Figure 4. From the with m/z 104, 103 and 78 is shown in Figure 5.
mass spectrum, base peak of m/z 104 was used for Method validation data is summarized in Table 2. Figures 6
quantitation where as m/z 103 and 78 were used as and 7 show overlay of SIM chromatograms for m/z 104 at
reference ions. linearity levels and calibration curve respectively.
SIM chromatogram of 50 ppb standard styrene solution

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