Page 37 - Pharmaceutical Solution for Pharma Analysis
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Reliable Analysis Even When Switching Between SFC and UHPLC Modes
SFC and UHPLC utilize significantly different mobile phases and separation characteristics, but analysis can be
performed reliably without effects from switching modes by simply purging the flow lines.
SFC (1) Switch from UHPLC (1) Switch from SFC (2) Switch from UHPLC (2)
SFC to UHPLC UHPLC to SFC SFC to UHPLC
SFC (n=5 for each) UHPLC (n=5 for each)
Propranolol
Indapamide Propranolol Indapamide
Ibuprofen
Ibuprofen
After switching from LC
(SFC (2)) After switching from SFC
(UHPLC (2))
Before switching After switching from SFC
(SFC (1)) (UHPLC (1))
1.0 2.0 min 1.0 2.0 min
Two Separation Methods Heighten the Efficiency of Examining the Optimal Separation Conditions
Various separation methods, including the separation of chiral compounds and structural isomer, are required in fields
such as pharmaceuticals, foods, and the environment. For example, in the case of method scouting with two chiral
standard samples, favorable separation is obtained for omeprazole with UHPLC conditions, and imdapamide with SFC
conditions. Screening utilizing these two methods makes it possible to construct better analysis conditions in a short
time. Switching between SFC and UHPLC analysis methods is easy with the dedicated software.
Best
UHPLC UHPLC UHPLC UHPLC UHPLC UHPLC
Chiral Column A Chiral Column B Chiral Column C Chiral Column A Chiral Column B Chiral Column C
Best
SFC SFC SFC SFC SFC SFC
Chiral Column A Chiral Column B Chiral Column C Chiral Column A Chiral Column B Chiral Column C
Omeprazole Method Scouting Imdapamide Method Scouting
Kit for Upgrading to an SFC Analysis System Shutoff Valve
Column Oven
Column
You can upgrade to an UHPLC/SFC switching system Solvent Delivery Unit Switching
(CO2 Pump)
Valve UV/PDA
(Nexera UC/s) capable of UHPLC and SFC analysis Solvent Delivery Unit Detector
using the existing* solvent delivery unit,
autosampler, oven, and detectors. Autosampler
Manifold
Back Pressure
Regulator
* The following units can be used in combination when upgrading.
Organic Solvent Mobile Phase
Solvent delivery Unit: LC-30AD
Solvent Delivery Unit
Autosampler: SIL-30AC
Column oven: CTO-20A/20AC Additional Unit
Detector: SPD-20A(V), SPD-M20A
Mass spectrometer: LCMS-2020, LCMS-80X0
Water Mobile Phase
© Shimadzu Corporation, 2017
First Edition: November 2016, Printed in Japan