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LAAN-A-TA-E016








            Application                  Thermal Analysis

            News                         Polymorphism of Drugs



            No.T152




            Q Introduction                                        DSC
                                                                  mW
            Polymorphism is the phenomenon of materials with the
            same chemical structure having different crystalline                               176.2 ˚C
            structures. The different crystal forms of polymorphic   0.0                     173.7 ˚C  189.5 ˚C
            materials have different properties, including solubility
            and stability, so evaluating polymorphism is an                                    175.1 ˚C
                                                                  -5.0
            important part of drug development. Differential                           heat of fusions: -106.6 J/g
            scanning calorimetry (DSC) is an effective technique for
            investigating polymorphism that requires no sample
                                                                 -10.0
            pretreatment and performs measurements quickly. DSC
            can be used to evaluate polymorphism based on
            different crystal shapes having different melting points                               190.8 ˚C
                                                                 -15.0
            and heat of fusions. Here, we describe an example use    50         100       150        200
            of DSC to analyze polymorphs.                                            Temp [˚C]
                                                                    Fig. 1  DSC Measurement of Carbamazepine Form ᶗ

            Q Measurement of Carbamazepine                        DSC
                                                                  mW
            Carbamazepine is used as an anticonvulsant and is
            known to exist in multiple crystal forms. Carbamazepine                               189.6 ˚C
            form ᶗ (Fig. 1) and form ᶙ (Fig. 2) were analyzed. A sharp   0.0
            endothermic peak can be observed with both forms at
            around 190 ˚C. This peak represents the melting of
            crystal form ᶙ. An endothermic/exothermic peak is     -5.0                  heat of fusions: -102.8 J/g
            visible between 170 ˚C and 180 ˚C with crystal form ᶗ.
            This phenomenon is speculated to be caused by
                                                                 -10.0
            recrystallization of formᶗ to stable form ᶙ following
            melting.
                                                                                                  190.7 ˚C
                                                                 -15.0
                                                                                100                  200
                                                                                     Temp [˚C]
                                                                    Fig. 2  DSC Measurement of Carbamazepine Form ᶙ
            Q Measurement of Sulfapyridine                        DSC
                                                                  mW
            Sulfapyridine was heated to 205 ˚C (first run), then
            cooled and heated again (second run) (Fig. 3).
            During the first run, only the melting peak of a      0.00  1st run   101.0 ˚C
            stabilized phase is visible at 192.2 ˚C, while during the
            second run, there is a glass transition at 54.7 ˚C, a      Onset   : 54.7 ˚C
                                                                       End-set  : 64.0 ˚C
            crystallization peak at 101 ˚C, and the melting peak of    Midpoint : 57.2 ˚C
            a quasi-stable phase at 181.9 ˚C.                    -10.00  2nd run              174.6 ˚C  192.1 ˚C


                                                                                                    192.2 ˚C
                                                                                                 181.9 ˚C
                                                                 -20.00
                                                                         50        100      150      200
                                                                                     Temp [˚C]
                                                                       Fig. 3  DSC Measurement of Sulfapyridine
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