Page 36 - Pharmaceutical Solution for Pharma Analysis

C190-E209

Upgrade Your Existing UHPLC

to an UHPLC/SFC Switching System

Reduce Instrument Purchase Costs and Enable SFC Analysis Immediately
You can now upgrade to an UHPLC/SFC switching system (Nexera UC/s) by adding the applicable SFC units to your
existing UHPLC system.
This enables you to perform both UHPLC and SFC analysis with a single system.

Upgrading Lowers
Purchasing Costs and
Reduces Installation Space

UHPLC System UHPLC/SFC Switching System (Nexera UC/s)

SFC Analysis Improves Separation and Analysis Times, while Reducing Solvent Consumption
In comparison to UHPLC, column efficiency is not impaired in SFC analysis even at a high flow rate. As a result, analysis
times are shortened by the increase in speed. At the same time, since the separation characteristics are different,
improved separation can be expected for foreign substances and isomers that are not sufficiently separated by UHPLC.
Further, the consumed amount of organic solvents can be reduced.

Improved Separation Shortened Analysis Times Reduced Solvent Consumption
with SFC Analysis
Hexane/
UHPLC 1. α-Tocopherol Isopropanol
ODS Column 2. β-Tocopherol 3 = 99:1
2 3 3. γ-Tocopherol 2 (Normal phase LC)
4. δ-Tocopherol 1 12.00 mL
4
1 Methanol
(Nexera UC) 0.70 mL 94.2% reduced
2 3
1
6.0 7.0 8.0 min 4
Comparison of organic solvent consumption
SFC
2 SFC-Amide Column UHPLC Normal phase LC 30,126 JPY
3
1
Nexera UC 3,745 JPY 87.6% reduced
SFC
2.0 3.0 4.0 min 0 2 4 6 8 10 12 min Comparison of costs