Page 19 - Shimadzu Prominence

Support for Ultra Fast Analysis

Switch from Conventional LC to Ultra Fast LC
Fast response is required to follow the sharp peaks obtained in ultra fast LC analysis. The 10 ms response of the RF-20A/20AXS permits
ultra fast LC analysis with no loss of separation. In this analysis example, the analysis time was reduced by a factor of more than three,
while maintaining the separation.
Conventional LC (N for Peak δ = 11,708)
α β γ δ
Analysis Conditions
Mobile phase Hexane / 2-propanol = 100 / 0.5 (v/v)
1.0 mL/min (Conventional)
Flow rate 12.2 min
0.8 mL/min (UFLC)
1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min
Shim-pack CLC-SIL(M) (150 mmL. × 4.6 mmI.D., 5 μm : Conventional)
Column UFLC (N for Peak δ = 10,623)
Shim-pack XR-SIL (75 mmL. × 3 mmI.D., 2.2 μm : UFLC) α δ
Temperature 30°C γ
Detection 298 nm excitation wavelength, 325 nm emission wavelength β
3.9 min
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min
Switching from Conventional LC to UFLC Analysis of α, β, γ, δ- Tochopherols
Multi-Component, High-Sensitivity UFLC Analysis
The highly sensitive simultaneous analysis of multiple components requires detection at the optimal wavelengths.
The RF-20A/20AXS permit ultra fast, high-sensitivity multi-component analysis using wavelength switching by time program.

A B C D E F G A 270 nm excitation wavelength, 330 nm emission wavelength
Naphthalene Acenaphthene Benzo[a]anthracene Benzo[k]fluoranthene Dibenz[a,h]anthracene Benzo[ghi]perylene B C 250 nm excitation wavelength, 370 nm emission wavelength
330 nm excitation wavelength, 430 nm emission wavelength
Fluorene Phenanthrene Anthracene Fluoranthene Chrysene Benzo[b]fluoranthene Benzo[a]pyrene Indeno[1, 2, 3-cd]pyrene D E 270 nm excitation wavelength, 390 nm emission wavelength
290 nm excitation wavelength, 430 nm emission wavelength
Pyrene
G F 370 nm excitation wavelength, 460 nm emission wavelength
270 nm excitation wavelength, 330 nm emission wavelength
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min
Ultra Fast Simultaneous Analysis of 15 Polycyclic Aromatics
Support for Improved Quantitative Analysis Accuracy

Utility of Four-Wavelength Measurement Function
Using detection at a single wavelength when performing each component at the optimal wavelength.
multicomponent simultaneous analysis of components with Detection using wavelength switching in the left-hand diagram
different optimal detection wavelengths necessitates sacrificing exhibits incomplete separation in area (1) and one peak of reduced
sensitivity for certain components. size in area (2). In such a case, setting up to four optimal
The RF-20A/20AXS detectors eliminate this issue by incorporating a wavelengths enhances the quantitative analysis accuracy by
four-wavelength measurement function that permits detection of reducing the effects of adjacent peaks and improving sensitivity.
mV mV
Wavelength-Switching λ1: Ex = 260 nm, Em = 350 nm Four-Wavelength Simultaneous 1) Ex=260nm Em=420nm
6000 λ2: Ex = 260 nm, Em = 420 nm 2) Ex=285nm Em=440nm
Chromatogram Measurement Chromatogram
λ3: Ex = 305 nm, Em = 495 nm 3) Ex=260nm Em=350nm
5000 7000 4) Ex=305nm Em=495nm
6000
4000
5000
3000
4000
2000 3000
2000
1000
1000
0
0
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0 0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
min min
Analysis of Polycyclic Aromatics by Four-Wavelength Simultaneous Measurement (Elution sequence shown in previous diagram)
19
High Performance Liquid Chromatograph