Page 17 - Shimadzu Prominence

High-Sensitivity Cell (Option)
A high-sensitivity cell with an 85 mm optical path length is available as an option for the SPD-M30A. It enables detection of trace
impurities that were conventionally difficult to detect and can be used for a wide range of analyses, from general analysis to ultra-fast and
UHPLC analysis.

Signal Intensity
High-sensitivity cell Standard cell
Impurity 1 49109 7931
Impurity 2 81339 11438
Impurity 3 16345 2290
Impurity 4 37922 5548
Impurity 5 7726 968

: Standard cell
: High-sensitivity cell
Impurity Analysis on Cefazolin Sodium (SPD-M30A)

85 mm Optical Path Length Significantly Enhances Sensitivity

The SPD-M30A high-sensitivity cell is also effective under HPLC conditions. The analysis below shows a comparison of measurements of
caffeine in a commercial soft drink using the SPD-M20A and SPD-M30A. It is apparent that the SPD-M30A improves the S/N ratio by up to
eleven times.

: SPD-M20A (Standard cell) SPD-M20A SPD-M30A S/N
3 : SPD-M30A (High-sensitivity cell) (Standard cell) (High-sensitivity cell) Relative Ratio
1 2794 26814 9.60
4 2 1527 15021 9.84
5
3 16153 179070 11.09
4 10894 118523 10.88
5 10394 113870 10.96
1 Mobile phase A : 0.1% aqueous solution of phosphoric acid
2
Mobile phase B : Acetonitrile
Flow rate : 1 mL/min
Column : Shim-pack VP-ODS 150 mmL. × 4.6 mmI.D.

New Analytical Techniques to Better Utilize PDA Detector Data

Separation of Unseparated Peaks (i-PDeA*) Extending the Dynamic Range (i-DReC**)
Area Area

Conc. Conc.

This technique fully separates unseparated peaks and visualizes small This powerful technique permits quantitation of samples in the
peaks hidden by a principal component. It is convenient when poor high-concentration range. It enables the simultaneous analysis of
separation occurs under conditions for accelerated analysis and for high- and ultra-low-concentration samples and the creation of
quantitation of small peaks overlapped by a principal component peak. calibration curves across a broad concentration range.

* i-PDeA: Intelligent Peak Deconvolution Analysis ** i-DReC: Intelligent Dynamic Range Extension Calculator
See the separate technical reports for more details about i-PDeA and i-DReC.

17
High Performance Liquid Chromatograph