Page 18 - Shimadzu Prominence

Fluorescence Detectors

The RF-20A/20AXS fluorescence detectors offer world-leading sensitivity* and
ease-of-maintenance. The RF-20AXS is a high-sensitivity model that incorporates a
temperature-controlled cell with a cooling function as standard.
RF-20A
Standard model
The RF-20A, which offers best-in-class sensitivity, features a water Raman S/N ratio of at
least 1200, as well as excellent ease-of-use with such features as maintenance from the
front panel and adoption of a long-life lamp.

RF-20AXS
Achieves World-Leading Sensitivity*
Offering world-class levels of sensitivity* and easy maintenance, the RF-20AXS features a water
Raman S/N ratio of at least 2000 and a temperature-controlled cell with a cooling function. This
maintains a constant detector cell temperature, even if the room temperature fluctuates
significantly, to ensure superb reproducibility with no drop in sensitivity. In addition, the RF-20AXS
incorporates an automatic wavelength accuracy check function using an internal low-pressure
mercury lamp to provide simple confirmation of the wavelength accuracy for validation.
mV
Achieves World-Leading Sensitivity * Anthracene

A powerful tool for the detection of ultra-trace components, the
RF-20Axs achieved a 21.5 S/N ratio for an injection of 10.48 fg
anthracene. This is equivalent to an approx. 1.5 fg detection limit
(S/N ratio = 3) and indicates superb sensitivity.

* As of August 2009, Survey by Shimadzu
High-Sensitivity Analysis of Anthracene
Cell Temperature Control Further Enhances Reproducibility (RF-20AXS)

The fluorescence intensity drops as the temperature rises. Room temperature 25°C Room temperature 25°C
A fluctuation of about 1°C near room temperature may result in Room temperature 30°C Room temperature 30°C
approximately 5% intensity fluctuations for some compounds.
To prevent this, the RF-20AXS features a temperature-controlled cell
with a cooling function. It maintains a constant detector cell
temperature, even if the room temperature fluctuates significantly, to
ensure superb reproducibility with no drop in sensitivity.
With cell temperature control No cell temperature control
Effect of Temperature-Controlled Cell (Acridine)

Rate of Change (%) %RSD %RSD Consecutive analysis is performed while changing the room temperature from 25°C to 30°C, and the %RSD value is
determined from the analysis data (n=6). It is used to confirm the effect of room-temperature fluctuations during the analysis.
RF-20AXS 0.64 0.29
(With cell temperature control) Rate of Change Consecutive analyses are performed at 25°C and 30°C room temperature. The rate of change shows the change in the peak
RF-20A area, taking the average peak area value at 25°C as 1. It is used to confirm the effect of long-term
(No cell temperature control) -17.45 6.30 fluctuations in room temperature due to the passage of the seasons.
Easy Maintenance
The Xenon lamp and flow cell can be replaced at the front panel. No positional
adjustment is required when replacing the Xenon lamp, and no tools are
required to replace the flow cell. The standard flow cell or semimicro flow cell
can be rapidly switched. In addition, the Xenon lamp life has been extended to
2000 hours, four times longer than previous Shimadzu lamps.

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