Page 9 - Shimadzu LCMS-8060

The triple quadrupole LCMS-8060 has an advanced ion guide design to increase ion production and detection and makes a meaningful
impact on high sensitivity detection.

High-Sensitivity Quantitation of Verapamil in Plasma

Verapamil in plasma; 100 ag on column
(%) Precision and accuracy of verapamil
ESI positive; m/z 455.40 > 165.00 CE: -27V
Expand your capabilities in quantitation Actual Conc. Calculated Conc. Area RSD Accuracy
(ng/mL)
(%, n=3)
(%)
(ng/mL)
Ultra sensitive detection %RSD 5.08% (n=10) 0.0001 0.000100 2.97 100.3
0.000508
0.0005
6.15
101.7
Linear dynamic range
0.1 pg/mL to 50 ng/mL 0.001 0.000942 5.32 94.3
0.00491
0.005
98.9
3.54
in bioanalysis 0.01 0.00949 2.94 95.0
0.05 0.0511 2.14 102.4
0.1 0.0996 1.18 99.8
Bringing together ultra fast scanning technologies 0.5 0.522 0.63 104.5
1 1.01 0.26 100.8
5 5.28 0.43 105.6
with high sensitivity pushes the limits of what we 10 10.0 0.60 100.0
50 48.8 0.33 97.8
can see.
• Verapamil spiked into a plasma crash sample can be quantified at 100 ag on column with a precision of 5.08% for multiple injections (n=10).
The LCMS-8060 is built for high data quality and • The LCMS-8060 also delivers a linear dynamic range from 0.1 pg/mL to 50 ng/mL.
drives confidence and consistency 24/7.
High-Sensitivity Quantitation of Steroid Hormones
300 1250
LLOQ level of aldosterone Aldosterone 1 pg/mL
250 (neat standard) 1000 (neat standard)
200
0.2 pg/mL 0.2 pg/mL 750 S/N 35
S/N 6.8 (peak to peak) LCMS-8060
150 Blank
(peak to peak) LCMS-8050
500
100 S/N 9.5
(peak to peak)
250
50
0 0
2.0 2.5 3.0 3.5 min 2.0 2.5 3.0 3.5 min
Actual conc. Mean area RSD Calc. conc. Accuracy
Lv
(pg/mL) (n=5) (n=5, %) (pg/mL) (%)
×10 7 1 0.2 448 13.2 0.190 94.8
6.00 2 0.5 1597 3.8 0.550 110.0

0.2–20000 pg/mL
5.00 Y=3187.32-156.16 3 1 3225 2.5 1.06 106.1
2
R > 0.9992 4 2 6085 1.4 1.96 97.9
4.00 16 levels 5 5 16398 2.0 5.19 103.9
3.00 6 10 31448 1.1 9.92 99.2
7 20 63339 1.2 19.9 99.6
2.00
8 50 158487 1.1 49.8 99.5
1.00 9 100 322774 0.9 101 101.3
10 200 602459 0.3 189 94.5
0.00 11 500 1556542 0.5 488 97.7
0 5000 10000 15000 Conc
12 1000 3284586 2.1 1031 103.1
• Aldosterone was detected at a lower concentration of 0.2 pg/mL 13 2000 5883876 0.5 1846 92.3
using neat standards and the calibration curve was linear from 0.2 14 5000 15641489 0.3 4907 98.1
15 10000 31567190 1.0 9904 99.0
to 20,000 pg/mL.
16 20000 59328173 2.6 18613 93.1

8 Liquid Chromatograph Mass Spectrometer LCMS-8060 9