Page 7 - Shimadzu LCMS-8060

Inspired by the need to balance advanced high speed MS/MS detection technologies with unrivalled LC Solving Complex Problems
performance, we were the first mass spectrometry company in the world to deliver a scan speed of 15,000 u/sec
Speed Solutions High-sensitivity quantitation of intact
and a polarity switching speed of 15 msec. In the LCMS-8060 the scan speed is now increased to 30,000 u/sec
catecholamines (CAs) in human plasma.
and a polarity switching speed of 5 msec making a real difference to working better and faster.
Sensitivity In clinical research, plasma catecholamines and their O-methylated
metabolites (metanephrines) are measured as biomarkers for
diseases such as hypertension, pheochromocytoma or
Loop time (msec) neuroblastoma.
Fast polarity switching 400 100 % Faster polarity switching time maximizes
Polarity Solutions
The LCMS-8060 uses UF switching dwell times and helps to optimize the
time It is a challenging assay as the low physiological levels of CAs,
Technologies to switch polarity cycle time of LC/MS/MS methods.
in 5 msec. Pause time physicochemical properties, and potential interferences require
high sensitivity and specificity.
Detect more 200 Dwell time Speed
325 Comparing the differed polarity switching times
295
Fast cycle time helps to detect (81%) (74%) from 5, 20 to 50 msec. We developed a SPE-LC/MS/MS assay by using LCMS-8060 to
235
more compounds with greater (59%) Ratio of dwell time (blue), pause time (red) and detect catecholamines at ultra-high sensitivity without matrix
confidence and precision. polarity switching time (green) in 400 msec of interferences. As the measurement cycle time was 12 mins

loop time when 65 of MRMs are simultaneously including the column re-equilibration the LCMS-8060 assay opens
monitored.
0 0 new possibilities for multiplexed sample analysis and higher
5 msec 20 msec 50 msec
Polarity switching time sample throughput.
×10 6
3.0 2 1. Norepinephrine
2. Epinephrine
×10 4 ×10 4 ×10 4
6.0 2.00 3. Dopamine
3.50 Positive Positive Negative Quantitative range of neat and matrix-matched calibration curves
1.75
m/z 398.1 > 313.9 LCMS-8060 5.0 m/z 367.0 > 127.1 LCMS-8060 m/z 213.0 > 141.0 LCMS-8060 Standard
3.00
Area RSD 4.55 % (n=5) ×5.10 Area RSD 4.76 % (n=5) ×4.57 1.50 Area RSD 3.41 % (n=5) ×3.79 2.0 Deuterated Neat standard curve Matrix-matched
2.50 4.0 Compound name
1.25 Range Linearity Range Linearity
3
2.00 (pg/mL) (r 2 ) (pg/mL) (r 2 )
3.0 1.00
1.50 0.75 1 Norepinephrine-d6 2.5 – 2000 0.9999 2.5 – 2000 0.9997
2.0 1.0 (158.1 > 111.1)
1.00 0.50
LCMS-8050 LCMS-8050 Epinephrine-d6
0.50 LCMS-8050 1.0 0.25 (190.1 > 172.1) 10 – 2000 0.9999 10 – 2000 0.9994
0.00 0.0 0.00 Dopamine-d4
9.25 9.50 9.75 10.00 10.25 10.50 min 9.25 9.50 9.75 10.00 10.25 10.50 min 9.25 9.50 9.75 10.00 10.25 10.50 0.0 (158.1 > 95.1) 5 – 2000 0.9999 10 – 2000 0.9995
Bensulide (SAP) Tetrachlorvinphos (CVMP) Mecoprop (MCPP)
1.0 2.0 3.0 4.0 min
Dwell time 2 msec, Loop time 400 msec
Representative MRM chromatograms
(×100,000) of 3 catecholamines
3.5
Norepinephrine Norepinephrine-d6 Epinephrine Epinephrine-d6 Dopamine Dopamine-d4
3.0 ×10 4 ×10 4 ×10 5 ×10 5 ×10 5 ×10 5
2.0 2.0
2.5
300 pg/mL 7.5 5.0 5.0
7.5 500 pg/mL
spiked spiked 500 pg/mL 1.5 1.5 500 pg/mL
2.0 spiked spiked
5.0 300 pg/mL
1.5 5.0
blank spiked 1.0 300 pg/mL 1.0
spiked
2.5 2.5
1.0 blank
2.5 2.5 0.5 0.5
0.5 blank blank blank blank
0.0 0.0 0.0
0.0 0.0 0.0 0.0
3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 min 0.5 2.5 min 0.5 2.5 min 1.0 2.5 min 1.0 2.5 min 2.5 5.0 min 2.5 5.0 min
MRM chromatograms of 105 pesticides (300 pg/mL each) using a polarity switching speed of 5 msec Detection of Norepinephrine, Epinephrine and Dopamine and their deuterated internal standards in plasma
and a sampling window of 65 compounds in 400 msec loop time
6 Liquid Chromatograph Mass Spectrometer LCMS-8060 7