Page 6 - Shimadzu LCMS-8060
P. 6

Inspired by the need to balance advanced high speed MS/MS detection technologies with unrivalled LC                                                         Solving Complex Problems
                             performance, we were the first mass spectrometry company in the world to deliver a scan speed of 15,000 u/sec
               Speed                                                                                                                    Solutions                                        High-sensitivity quantitation of intact
                             and a polarity switching speed of 15 msec. In the LCMS-8060 the scan speed is now increased to 30,000 u/sec
                                                                                                                                                                                         catecholamines (CAs) in human plasma.
                             and a polarity switching speed of 5 msec making a real difference to working better and faster.
                                                                                                                                                          Sensitivity                    In clinical research, plasma catecholamines and their O-methylated
                                                                                                                                                                                         metabolites (metanephrines) are measured as biomarkers for
                                                                                                                                                                                         diseases such as hypertension, pheochromocytoma or
                                              Loop time (msec)                                                                                                                           neuroblastoma.
            Fast polarity switching        400                             100 %    Faster polarity switching time maximizes
                                                                   Polarity                                                                                  Solutions
            The LCMS-8060 uses UF                                  switching     dwell times and helps to optimize the
                                                                   time                                                                                                                  It is a challenging assay as the low physiological levels of CAs,
            Technologies to switch polarity                                      cycle time of LC/MS/MS methods.
            in 5 msec.                                             Pause time                                                                                                            physicochemical properties, and potential interferences require
                                                                                                                                                                                         high sensitivity and specificity.
            Detect more                    200          Dwell time                                                                                                   Speed
                                                  325                        Comparing the differed polarity switching times
                                                           295
            Fast cycle time helps to detect      (81%)     (74%)             from 5, 20 to 50 msec.                                                                                      We developed a SPE-LC/MS/MS assay by using LCMS-8060 to
                                                                     235
            more compounds with greater                             (59%)    Ratio of dwell time (blue), pause time (red) and                                                            detect catecholamines at ultra-high sensitivity without matrix
            confidence and precision.                                        polarity switching time (green) in 400 msec of                                                              interferences. As the measurement cycle time was 12 mins

                                                                             loop time when 65 of MRMs are simultaneously                                                                including the column re-equilibration the LCMS-8060 assay opens
                                                                             monitored.
                                             0                             0                                                                                                             new possibilities for multiplexed sample analysis and higher
                                                 5 msec   20 msec   50 msec
                                                      Polarity switching time                                                                                                            sample throughput.
                                                                                                                                                 ×10 6
                                                                                                                                               3.0             2   1. Norepinephrine
                                                                                                                                                                   2. Epinephrine
                    ×10 4                         ×10 4                          ×10 4
                                                6.0                           2.00                                                                                 3. Dopamine
                  3.50 Positive                    Positive                       Negative                                                                                               Quantitative range of neat and matrix-matched calibration curves
                                                                              1.75
                     m/z 398.1 > 313.9   LCMS-8060  5.0  m/z 367.0 > 127.1  LCMS-8060  m/z 213.0 > 141.0  LCMS-8060                                                     Standard
                  3.00
                     Area RSD 4.55 % (n=5)    ×5.10  Area RSD 4.76 % (n=5)    ×4.57  1.50  Area RSD 3.41 % (n=5)   ×3.79                       2.0                      Deuterated                      Neat standard curve  Matrix-matched
                  2.50                          4.0                                                                                                                                        Compound name
                                                                              1.25                                                                                                                      Range  Linearity  Range  Linearity
                                                                                                                                                                      3
                  2.00                                                                                                                                                                                  (pg/mL)  (r 2 )  (pg/mL)  (r 2 )
                                                3.0                           1.00
                  1.50                                                        0.75                                                                          1                              Norepinephrine-d6  2.5 – 2000  0.9999  2.5 – 2000  0.9997
                                                2.0                                                                                            1.0                                          (158.1 > 111.1)
                  1.00                                                        0.50
                                                               LCMS-8050                      LCMS-8050                                                                                     Epinephrine-d6
                  0.50            LCMS-8050     1.0                           0.25                                                                                                          (190.1 > 172.1)  10 – 2000  0.9999  10 – 2000  0.9994
                  0.00                          0.0                           0.00                                                                                                          Dopamine-d4
                      9.25  9.50  9.75  10.00  10.25  10.50 min  9.25  9.50  9.75  10.00  10.25  10.50  min  9.25  9.50  9.75  10.00  10.25  10.50  0.0                                     (158.1 > 95.1)  5 – 2000  0.9999  10 – 2000  0.9995
                            Bensulide (SAP)           Tetrachlorvinphos (CVMP)         Mecoprop (MCPP)
                                                                                                                                                    1.0    2.0    3.0    4.0  min
                                                 Dwell time 2 msec, Loop time 400 msec
                                                                                                                                                     Representative MRM chromatograms
                    (×100,000)                                                                                                                             of 3 catecholamines
                  3.5
                                                                                                                                          Norepinephrine  Norepinephrine-d6  Epinephrine    Epinephrine-d6    Dopamine        Dopamine-d4
                  3.0                                                                                                                    ×10 4            ×10 4           ×10 5            ×10 5           ×10 5            ×10 5
                                                                                                                                                                                                         2.0             2.0
                  2.5
                                                                                                                                          300 pg/mL    7.5              5.0             5.0
                                                                                                                                       7.5                500 pg/mL
                                                                                                                                          spiked          spiked                           500 pg/mL     1.5             1.5      500 pg/mL
                  2.0                                                                                                                                                                      spiked                                 spiked
                                                                                                                                                       5.0                 300 pg/mL
                  1.5                                                                                                                  5.0
                                                                                                                                          blank                            spiked                        1.0     300 pg/mL  1.0
                                                                                                                                                                                                                 spiked
                                                                                                                                                                        2.5             2.5
                  1.0                                                                                                                                     blank
                                                                                                                                       2.5             2.5                                               0.5             0.5
                  0.5                                                                                                                                                       blank           blank           blank           blank
                  0.0                                                                                                                  0.0             0.0
                                                                                                                                                                        0.0             0.0              0.0             0.0
                    3.0    4.0     5.0     6.0     7.0     8.0     9.0     10.0    11.0    12.0   13.0  min                             0.5     2.5 min  0.5     2.5 min  1.0  2.5  min   1.0   2.5  min   2.5       5.0 min  2.5     5.0 min
                            MRM chromatograms of 105 pesticides (300 pg/mL each) using a polarity switching speed of 5 msec                         Detection of Norepinephrine, Epinephrine and Dopamine and their deuterated internal standards in plasma
                                        and a sampling window of 65 compounds in 400 msec loop time
      6                                                                                                                                                                                                    Liquid Chromatograph Mass Spectrometer  LCMS-8060  7
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