Page 35 - Application Notebook - PFAS Analysis

No. SSI-LCMS-106

6-2 FTS 5.797 427 > 407 23
427 >81 39
M6-2 FTS 5.799 429 >409 22
PFOA 6.048 413 > 369 10
413 >169 17
M8PFOA 6.051 421 > 376 10
PFHxS 6.305 399 > 80 43
399 >99 22
M3PFHxS 6.306 402 > 80 49
403 >84 49
PFNA 6.642 463 > 419 11
463 >219 16
M9PFNA 6.641 472 > 427 12
8-2 FTS 6.927 527 > 507 26
527 >81 49
M8-2 FTS 6.928 529 > 509 26
527 >81 49
PFHpS 6.928 449 > 80 51
449 >99 37
N-MeFOSAA 7.254 570 > 419 21
570 >483 16
d3M N-MeFOSAA 7.243 573 > 419 20
PFDA 7.189 513 > 468.9 11
413 >219 17
M6PFDA 7.188 519 > 474 11
N-EtFOSAA 7.469 584 > 419 20
584 >483 16
M N-EtFOSAA 7.463 589 > 419 21
PFOS 7.483 499 > 80 54
499 >99 38
M8PHOS 7.484 507 > 80 55
PFUdA 7.697 563 > 519 12
563 >269 16
M7PFUdA 7.695 570 > 525 12
PFNS 8.009 549 > 80 54
549 >99 44
PFDoA 8.181 613 > 569 12
613 >169 21
MPFDoA 8.179 615 > 570 11
FOSA 8.498 498 > 78 43
M8FOSA 8.498 506 > 78 48
PFDS 8.523 599 > 80 55
599 >99 50
PFTriA 8.662 663 > 619 12
663 >169 27
PFTeDA 9.155 713 > 669 13
713 >169 27
M2PFTeDA 9.130 715 > 670 15

Calibration Standards Sample Preparation
Standards available from Wellington Laboratories EPA Method 8327 was tested using reagent water,
were used for these studies (Catalog no. PFAC- surface water, ground water, and wastewater as
24PAR and MPFAC-24ES). These standards were sample matrices. This report outlines data collected
then diluted to working standards as outlined in with representative chromatograms and tables for
Section 7.4 of EPA Method 8327 using 95:5 each matrix tested. Each sample was diluted 50:50
acetonitrile:water as the diluent. The working with MeOH and 0.1% acetic acid, spiked with
standards were used to create a calibration curve isotopically labeled surrogates and vortexed for 2
ranging from 5-200 ppt with the injection solvent min. The samples were then filtered through /0.2 μm
consisting of 50:50 water:methanol with 0.1% syringe filters and analyzed by LC/MS/MS.
acetic acid in order to match the injection solvent for
the extracted samples. Filtration was not performed
on the calibration standards