Page 24 - Microorganism Species Analysis

Prominence UFLC Prominence UFLC
Analysis of Amino Acids, Organic Acids, and Aflatoxins
Microorganism Solutions Microorganism Solutions

UFLC Analysis of Microorganism-Derived Components Data
High-performance liquid chromatography is widely used for the analysis of amino acids in fermented and brewed food
products. However, the demand for fast analysis has been increasing over recent years to enhance the productivity of Fast Analysis of Amino Acids by Pre-Column Derivatization
laboratory analysis. The Prominence Ultra Fast Liquid Chromatograph (UFLC) is based on a comprehensive analysis of
Derivatization Reaction with Phenylisothiocyanate (PITC) Microorganism Species Observation of
technologies to increase the speed of liquid chromatography. In addition to ultra-fast liquid chromatography, it achieves high Standard sample
1. To 200 µL sample, add 100 mmol/L phenylisothiocyanate
levels of accuracy, stability, durability, and expandability not available from conventional HPLC. The ultra-fast analysis and
(acetonitrile solution) and 1 mol/L triethylamine (acetonitrile
high accuracy and reliability offered by the Prominence UFLC achieve revolutionary improvements in laboratory analysis
Observation of
solution), agitate, and leave at 40 °C for 20 minutes to react.
productivity.
2. Allow to cool to room temperature, and spike with 1.2 mol/L
Microorganism Species
Commercial soy sauce
hydrochloric acid aqueous solution to neutralize.
This is an example of the fast analysis of amino acids by
Features of the Prominence UFLC Ultra Fast Liquid Chromatograph
pre-column derivatization using PITC. Analysis was performed on
Ultra-fast LC analysis: just one-tenth the analysis time of general LC amino acids, 23 related components (0.5 µmol/L), and commercially
Shorter total analysis cycle time available soy sauce. The analysis was completed within 10
High-resolution analysis: three times the resolution of general LC minutes.
Analytical Conditions Microorganism Species Identification of
High accuracy, stability, and durability Column : Shim-pack XR-ODS (100 mm L. × 3.0 mm I.D.)
Mobile phase : A) 10 mmol/L (potassium) phosphate buffer (pH7.0)
The Shim-pack XR-ODS Column for fast analysis and high separation maximizes the Prominence UFLC B) Acetonitrile
performance. B.conc; 5% (0 to 0.5 min), 5% to 35% (0.5 to 10.5 min)
Flow rate : 0.9 mL/min
Identification of
Column temp. : 40 °C
Detection : absorbance at 254 nm with semi-micro flow cell
Microorganism Species
Sample volume : 1 µL
Fast Analysis of Organic Acids

mAU The ion exclusion mode is often used for the analysis of organic
8 1 acids, but in this case an ODS column that can handle 100%
2 4
7 Specific Microorganisms Detection of
aqueous solutions was used to complete fast analysis within two
6
minutes.
5 3
4
Analytical Conditions
Detection of
3
Column : Phenomenex Synergi 2.5 µm Hydro-PP 100A (100 mm L. × 3.0 mm I.D., 2.5 µm)
2 Mobile phase : 10 mmol/L (sodium) phosphate buffer (pH2.6)
1 Flow rate : 0.8 mL/min
Specific Microorganisms
0 Column temp. : 30 °C
Injection volume : 4 µL
0.00 0.25 0.50 0.75 1.00 1.25 1.50 1.75 min
Detection : SPD-20A at 210 nm
Flow cell : semi-micro cell
Fast Analysis of Cephem Antibiotics
Analytical Conditions
Prominence UFLC Column : Shim-pack XR-ODS Components Analysis of Microorganism-Derived
(100 mm L. × 3.0 mm I.D., 2.2 µm)
Shim-pack VP-ODS
(250 mm L. × 4.6 mm I.D., 4.6 µm)
Components
Mobile phase : A:0.1% formic acid-water
B:acetonitrile
Time program [XR-ODS]
B.conc; 15% (0 min)
55% (3.5 min)
15% (3.51-6.5 min)
Analysis of Microorganism-Derived
[VP-ODS] Structure of Cephem Antibiotics (Partial)
B.conc; 15% (0 min)
55% (30 min)
15% (30.01-45 min); 0.2 mL/min Cephem antibiotics are a type of beta-lactam
Flow rate : 1.0 mL/min (XR-ODS) antibiotic. They are broad-spectrum
1.0 mL/min (VP-ODS)
Column temp. : 40 °C antibiotics with a strong antibiotic action, and
Injection volume: 4 µL (XR-ODS)
10 µL (VP-ODS) are used as general-purpose oral or injected
Detection : photodiode array antibiotics. The combination of Prominence Other Applications
UV-VIS detector 260 nm
Flow cell : semi-micro cell (XR-ODS) UFLC and Shim-pack XR-ODS increases the
conventional (VP-ODS)
mobile phase linear velocity to approximately
2.4 times that of the Shim-pack VP-ODS
Other Applications
during analysis. In addition, it maintains high
LCsolution Screen Shim-pack XR-ODS Chromatogram of 13 Cephem Antibiotic Components resolution while reducing the analysis cycle
Column for Fast Analysis and High Separation (Upper: Shim-pack VP-ODS; time by approximately 85%.
Lower: Shim-pack XR-ODS)
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