Page 21 - Microorganism Species Analysis

GCMS-QP2010 Plus GCMS-QP2010 Plus
Analysis of Amino Acids, Fatty Acids, and Organic Acids
Microorganism Solutions Microorganism Solutions

GC/MS Analysis of Microorganism-Derived Components Data
Shimadzu and the Faculty of Medicine, Shimane University, jointly developed the GC/MS Metabolite Component Database,
which contains over 300 metabolites (amino acids, fatty acids, organic acids), using a GCMS-QP2010 Plus Gas Fast Analysis of Amino Acids Using the GC/MS Metabolite Component Database
Chromatograph-Mass Spectrometer (GC/MS). A combination of the GC/MS Metabolite Component Database and a GC/MS
The GC/MS Metabolite Component Database offers optimal column and consumables information, method files Microorganism Species Observation of
permits the batch analysis of biological metabolite components.
containing suitable analytical conditions and compound information, and libraries with retention indices. The
metabolite database targets amino acids, fatty acids, and organic acids. It offers four mass spectrum libraries
Observation of
containing retention indices and mass spectra for these derivatives and four method files containing data analysis
conditions (Table 1).
Features of the Biological Component Analysis System Using the
Microorganism Species
Combination of GC/MS and the GC/MS Metabolite Component EZ:faast™ contains reagents for amino acid analysis pretreatment and derivatization, amino acid standard solution
(Amino Acids, Fatty Acids, Organic Acids) Database (33 components), pretreatment equipment and an analysis capillary column kit.
Sample pretreatment and derivatization is completed in seven steps (taking approximately seven minutes). In addition
A system comprised of the GCMS-QP2010 Plus and the GC/MS Metabolite Component Database is ideal for
to the 33 components above, this kit supports over 50 amino acids and related compounds.
research into biological metabolite components, targeting amino acids, fatty acids, and organic acids.
The database contains not only GC retention indices, mass spectra, and compound information for over 300 Analytical Conditions Table 1 Method Files and Libraries Contained in the Database
metabolites but also analysis method files and data analysis conditions for the target components. It allows fast and Model : GCMS-QP2010, GCMS-QP2010 Plus (high-power oven) Microorganism Species Identification of
Workstation : GCMSsolution Ver. 2.5
easy detection and identification of biological metabolite components, with fewer investigations of analysis Column : ZB-AAA 10 m × 0.25 mm I.D. Method file/mass Derivatization Ionization No. of spectra
spectrum library
method
method
conditions and reduced setup work. — GC — — MS —
Inj. temp. : 280 °C Interface temp. : 280 °C
The EZ:faast™ Reagent Kit* simplifies derivatization pretreatment of samples for amino acid analysis. Column temp. : 110 °C (0 min) 30 °C/min Ion source temp. : 200 °C Amino acid Note) EZ:faast™ Electron ionization (EI) 33
Identification of
-320 °C (0 min) Scan range : m/z : 45-450
* EZ:faast™ is manufactured by Phenomenex Inc. Carrier gas : He Scan interval : 0.15 sec
Microorganism Species
Flow control mode : pressure Fatty acid Methylation Electron ionization (EI) 50
Pressure : 15 kPa
Injection method : split Fatty acid Methylation Chemical ionization (CI) 50
Split ratio : 1:15
Organic acid/ Trimethylsilylation Electron ionization (EI) 178
amino acid
Derivatization Data analysis and database research
Note) As derivatization methods and libraries using the EZ:faast™ kit require
rapid heating rates in the column oven, measurements are performed on a
230 V-specification instrument (high-power oven). Specific Microorganisms Detection of
(x1,000,000)
TIC
4.0 31
Detection of
3.5
Specific Microorganisms
3.0
29
2.5
20
2.0
25
EZ:faast™ kit 7 9 17
1.5 5 15 22
4 8 10 13 16
1.0 1 2 6 11 14 21 24 26 27 30 32 33
3 12 18 19 28
GCMSsolution Ver. 2 Series 0.5 23 Components Analysis of Microorganism-Derived
GC/MS Workstation
1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
Components
Mass spectrometry
Total Ion Chromatogram of 33-Component Amino Acid Standard Solution
Analysis of Microorganism-Derived
Estimated and Actual Retention Times for 33 Amino Acid Components
Retention time Retention time
ID Name R.I. Estimated value Actual value Difference ID Name R.I. Estimated value Actual value Difference
1 Alanine 1675 1.189 1.187 -0.002 18 4-Hydroxyproline 2249 2.923 2.929 0.006
2 Sarcosine 1697 1.249 1.248 -0.001 19 Glutamic acid 2344 3.193 3.191 -0.002
3 Glycine 1712 1.294 1.293 -0.001 20 Phenylalanine 2352 3.215 3.217 0.002
4 alpha-aminobutyric acid 1748 1.401 1.4 -0.001 21 alpha-Aminoadipic acid 2463 3.518 3.519 0.001
5 Valine 1781 1.5 1.498 -0.002 22 alpha-Aminopimelic acid 2569 3.797 3.798 0.001
6 beta-Aminoisobutyric acid 1806 1.575 1.574 -0.001 23 Glutamine 2598 3.872 3.875 0.003
7 Norvaline 1825 1.634 1.631 -0.003 24 Ornithine 2767 4.292 4.295 0.003 Other Applications
8 Leucine 1853 1.721 1.718 -0.003 25 Glycine-proline (dipeptide) 2785 4.335 4.343 0.008
9 allo-Isoleucine 1862 1.749 1.747 -0.002 26 Lysine 2885 4.57 4.572 0.002
10 Isoleucine 1872 1.78 1.779 -0.001 27 Histidine 2977 4.782 4.777 -0.005
11 Threonine 1943 2 1.998 -0.002 28 Hydroxylysine (2 isomers) 3059 4.965 4.958 -0.007
12 Serine 1956 2.04 2.04 0 29 Tyrosine 3107 5.071 5.072 0.001
13 Proline 1980 2.114 2.117 0.003 30 Proline-hydroxyproline (dipeptide) 3218 5.306 5.307 0.001
Other Applications
14 Asparagine 2014 2.219 2.22 0.001 31 Tryptophan 3252 5.376 5.386 0.01
15 Thiaproline 2137 2.594 2.597 0.003 32 Cystathionine 3509 5.901 5.89 -0.011
GCMS-QP2010 Plus
16 Aspartic acid 2209 2.807 2.807 0 33 Cystine 3626 6.14 6.128 -0.012
Gas Chromatograph - Mass Spectrometer GC/MS Metabolite Component Database 17 Methionine 2219 2.836 2.839 0.003
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