Page 22 - Microorganism Species Analysis

Prominence HPLC Prominence HPLC
Analysis of Saccharides, Organic Acids, and Amino Acids
Microorganism Solutions Microorganism Solutions

LC Analysis of Microorganism-Derived Components Data
The high-performance liquid chromatograph is widely used for the analysis of microorganism-derived components. Due to
the diversity of microorganism-derived components, a variety of detectors and applications are required to analyze them with Analysis of Amino Acids Analysis of Saccharides in Brewed Products
high sensitivity and high accuracy. (Fluorescence Detector) (Spectrofluorometric Detector) Microorganism Species Observation of
Shimadzu supplies a range of detectors from UV-VIS detectors to mass spectrometers to provide the optimal system.
The Shimadzu post-column reaction method uses Due to its selectivity and sensitivity, post-column
derivatization offering selective reaction with amino bases fluorescence derivatization is suitable for the analysis of
Observation of
and spectrofluorometric detection to deliver at least an saccharides in brewed products.
order of magnitude greater detection sensitivity than the This is an example of the batch analysis of saccharides
Features of the Prominence High-Performance Liquid Chromatograph
Microorganism Species
ninhydrin method with UV detection. in Japanese sake with Shimadzu’s Reducing Sugar
The Prominence High-Performance Liquid Chromatograph offers superior functionality and performance than Analysis System, which uses a unique arginine reagent.
conventional LC instruments, with such features as Web control functions and high-sensitivity detection.
Fully automated analysis, self-diagnostics and auto-recovery (Expert functions), and Web control functions
significantly enhance the analysis productivity compared to conventional instruments.
Detector sensitivity, linearity, and baseline stability are important to improve the reliability of purity testing.
The Prominence HPLC offers high sensitivity, superb linearity, and a stable baseline due to ultra-low-pulsation Microorganism Species Identification of
pumping to provide powerful support for purity testing.
The LCsolution workstation for Prominence offers comprehensive system management functions, FDA 21 CFR part
11-compatible security, and electronic signatures. Analysis of Amino Acid Components
Identification of
Select the optimum LC column from the wide range of columns from Shimadzu GLC Ltd.
Analytical Conditions
Microorganism Species
Column : Shim-pack Amino-Na Analysis of Sake
(100 mm L. × 6.0 mm I.D.)
Ammonia trap : Shim-pack ISC-30/S0504Na Analytical Conditions
(50 mm L. × 4.0 mm I.D.) Column : Shim-pack ISA-07/S2054 (250 mm L. × 4.6 mm I.D.)
Mobile phase : Amino Acid Mobile Phase Kit Guard column : ISA (50 mm L. × 4.6 mm I.D.)
(Na type), gradient elution Mobile phase : A: 0.1 M (potassium) borate buffer (pH8)
Flow rate of mobile phase : 0.4 mL/min B: 0.4 M (potassium) borate buffer (pH9)
Column temp. : 60 °C A B / linear gradient elution
Reaction reagent : Amino Acid Reagent Kit Flow rate : 0.6 mL/min
Flow rate of reaction reagent : 0.2 mL/min, each Column temp. : 65 °C
Reaction temp. : 60 °C Reagent : 1% arginine, 3% boric acid
Detection : Ex at 350 nm, Em at 450 nm Flow rate of reagent : 0.5 mL/min Specific Microorganisms Detection of
Reaction temp. : 150 °C
Detection : Ex at 320 nm, Em at 430 nm
Detection of
Analysis of Saccharides in Bioethanol Analysis of Organic Acids
Specific Microorganisms
Production (ELSD Detector) (Electric Conductivity Detector)
Bioethanol is produced by fermenting saccharides derived Absorptiometric detection is used for the analysis of
from biomass materials, such as sugar cane or maize. organic acids. However, as the detection wavelength is
This is an example of the analysis of saccharides related near the 205 nm peak derived from the absorption of the
to bioethanol production using the ELSD-LT II Evaporative carboxyl group, this method is susceptible to effects from
Prominence Amino Acid Analysis System Prominence Reducing Sugar Analysis System
(with Fluorescence Detector) (with Spectrofluorometric Detector) Light Scattering Detector. impurities and some samples require complex
pretreatment. In some cases analysis is extremely difficult. Components Analysis of Microorganism-Derived
This is an example of Peaks
the analysis of beer 1. Phosphoric acid
Components
2. Citric acid
3. Pyruvic acid
using an electric 4. Malic acid
5. Succinic acid
conductivity detector, 6. Lactic acid
7. Formic acid
which is highly 8. Acetic acid
Analysis of Microorganism-Derived
9. Levulinic acid
sensitive and selective
for ionic substances.
Analysis of Xylo-Oligosaccharides
Analytical Conditions Analytical Conditions Analysis of Beer Other Applications
Column : Unison UK-Amino (250 mm L. × 4.6 mm I.D., 3 µm) For separation
Mobile phase : A: water, B: acetonitrile Column : Shim-pack SCR-102H (300 mm L. × 8.0 mm I.D.)
B Conc. 90% (0 min) 90% (5 min) 55% (30 min) Mobile phase : 5 mM p-toluenesulfonic acid
30% (31–36 min) 90% (36.01–51 min) Flow rate : 0.8 mL/min
Flow rate : 1.0 mL/min Temperature : 45 °C
Other Applications
Prominence Organic Acid Analysis System Column temp. : 25 °C For detection
Prominence Analysis System (with ELSD Detector) (with Electric Conductivity Detector) Injection volume : 10 µL Reagent : 5 mM p-toluenesulfonic acid,
Detection : ELSD-LT II 20 mM Bis-Tris and 100 µM EDTA
Temperature : 40 °C Nebulizing gas : N2 Flow rate : 0.8 mL/min
Gain : 6 Gas pressure : 350 kPa Temperature : 48 °C
Detection : electric conductivity
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