Page 23 - Application Handbook - Liquid Chromatography

Application No.L462
News

n Comprehensive Separation of Glycerophospholipids The GPLs mixture was comprised of 500 ppb each of
Comprehensive Separation of GPLs in ESI-positive MRM Phosphatidylglycerol (PG), Phosphatidylethanolamine
mode are shown in Fig. 2. (PE), Phosphatidylinositol (PI), Phosphatidylserine (PS)
and Phosphatidylcholine (PC). The 2D plot of ESI-
positive MRM shows the separation of PG, PE, PI, PS
and PC. The repeatability (n=5) of retention times and
blob areas, which correspond to peak areas in ordinary
quantitation and linearity for 50-5000 µg/L of each 3
PC compounds are is shown in Table 2.
Necessary information for compound-identification is
shown in Fig. 2.

(min.)
0.981
PE group
2D 18:0p-18:1 PC
0.785
18:0-18:1 PC

0.589
16:0-16:0 PC
PI group
PS group
0.392
PC group
0.196
PG group
1D
100
100 13.998 27.997 41.995 55.994 69.992 (min.)

Area
750000000.0

500000000.0

250000000.0

0.0
0 500 1,000 1,500 2,000 2,500 3,000 3,500 4,000 4,500 5,000
Conc. (µg/L)

Fig. 2 Comprehensive Separation and Calibration Curve of GPLs

Table 2 Repeatability of 5 Analyses in %RSD and Linearity of 50-5000 μg/L for 3 PC Compounds
Compound MRM transition Total retention time Retention time (2D) Blob Area Correlation coefficient (R)
16 : 0 -16 : 0 PC m/z 734.6 > 184.1 0.0072 0.9 6.8 0.999799
18 : 0 -18 : 1 PC m/z 788.6 > 184.1 0.013 1.1 8.9 0.999947
18 : 0p -18 : 1 PC m/z 772.6 > 184.1 0.013 1.2 6.4 0.999656

First Edition: Jun. 2014

For Research Use Only. Not for use in diagnostic procedures.
The content of this publication shall not be reproduced, altered or sold for any commercial purpose without the written approval of Shimadzu.
The information contained herein is provided to you "as is" without warranty of any kind including without limitation warranties as to its
accuracy or completeness. Shimadzu does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the
use of this publication. This publication is based upon the information available to Shimadzu on or before the date of publication, and subject
to change without notice.
www.shimadzu.com/an/ © Shimadzu Corporation, 2014