Page 103 - Application Handbook - Liquid Chromatography

Application No.L456
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n Linearity of Calibration Curves
Each counter cation was used for four standard
mixtures in the concentration range from 1.25 to
10 mg/L. Then measurements were done using an
electrocoductivity detector to create calibration curves,
which are shown in Fig. 2.

a) b) c) d)
Area
Area Area 250000 Area
150000 Na 80000 K Mg 140000 Ca
R =0.999 R =0.999 R =0.999 R =0.999
2
2
2
2
200000 120000
60000
100000
100000
150000
80000
40000
100000 60000
50000
20000 40000
50000
20000
0 mg/L 0 mg/L 0 mg/L 0 mg/L
0.0 2.5 5.0 7.5 10.0 0.0 2.5 5.0 7.5 10.0 0.0 2.5 5.0 7.5 10.0 0.0 2.5 5.0 7.5 10.0
Fig. 2 Linearity of Calibration Curves
a) Sodium, b) Potassium, c) Magnesium, d) Calcium
n Analysis of Counterions
Fig. 3 shows the results of analysis of a standard The mole ratios of the principal components and
solution of diclofenac sodium (44.7 mg/L : 0.1 mmol/L) counterions were diclofenac : sodium = 1:1.1, and
containing sodium salt, and Fig. 4 shows the results of benzylpenicillin : potassium = 1:1.
analysis of a standard solution of benzyl penicillin In addition, the area repeatability obtained in repeat
(37.2 mg/L : 0.1 mmol/L) containing potassium salt. The analysis (n=6) of each of the standard solutions was
analytical conditions are shown in Table 2. The 0.02 % for sodium in diclofenac, and 0.09 % for
quantitative values obtained for the counterions were potassium in benzylpenicillin, indicating excellent
2.4 mg/L for sodium (0.1 mmol/L), and 3.9 mg/L repeatability in both analyses.
(0.1 mmol/L) for potassium.

uV
uV
˙Peak 35000 ˙Peak
50000 1. Na 1. K
30000
40000 1 %RSD = 0.02 % 25000 1
%RSD = 0.09 %
30000 20000
15000
20000
10000
10000
5000
0
0
0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 min

Fig. 3 Chromatogram of Diclofenac Sodium Fig. 4 Chromatogram of Benzylpenicillin Potassium

First Edition: May. 2014

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