Page 107 - Application Handbook - Liquid Chromatography

Application No.L504
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n Calibration Curve Linearity
A standard sample of fumaric acid ions was used to
prepare four standard concentrations in the range of
6.25 to 50 mg/L. The calibration curve created using
these standard concentrations and a UV-VIS detector is
shown in Fig. 2. As shown in Fig. 2, good linearity was
obtained.

Area (×10 ) Fig. 3 Structural Formula of Clemastine Fumarate
6
4.0 Fumaric acid
2
R = 0.99998
mV
3.0
60 1
■Peak
1. Fumaric acid
2.0 50

40
1.0
30
0.0
0 10 20 30 40 mg/L 20

Fig. 2 Calibration Curve Linearity 10

0
n Analysis of Counterions 0 5 10 15 20 25 30 min
The structural formula of the antihistamine drug
clemastine fumarate is shown in Fig. 3, and an example
analysis of a standard solution of clemastine fumarate Fig. 4 Chromatogram of Clemastine Fumarate
(50 mg/L: 0.11 mmol/L) is shown in Fig. 4. The
analytical conditions used are identical to those shown
in Table 1. Table 2 Results of Quantitative Analysis of Fumaric Acid Ions
The results of quantitative analysis of the fumaric acid Quantitative Value [mg/L] 13.20
ions present in the clemastine fumarate standard Theoretical Value [mg/L] 12.62
solution are shown in Table 2. Quantitative analysis Recovery Rate % 104.6
showed the presence of 13.20 mg/L (0.029 mmol/L) of
fumaric acid ions, which is a good recovery rate of
104.6 % when compared to the theoretical value of
12.62 mg/L.

First Edition: Apr. 2016
For Research Use Only. Not for use in diagnostic procedure.
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