Page 17 - Pharmaceutical- Guide to Biopharmaceutical

Culture

Cell Culture Media Analysis Platform C2MAP Cell Culture Media Analysis Platform System

Automates Processes from Pretreatment to LC/MS/MS benefits

Measurement for Culture Supernatant Analysis Cell Line Optimization
click here • Automating culture supernatant analysis processes from pretreatment to LC/MS
measurement ensures anyone can acquire high-quality data.
Operating Principle and Features
• Dedicated control software makes it easy to link samples to measurement results.
The C2MAP-2030 is an automatic pretreatment system for removing TRENDS software included with the C2MAP-2030 system. • The system can be operated using only the modules necessary for automatic
proteins from culture supernatants by suction filtering the proteins By connecting the C2MAP automatic pretreatment system to the LC-
precipitated by adding an organic solvent. Deproteinized culture MS/MS system, samples can be analyzed seamlessly. Because sample pretreatment, while the LC-MS/MS system is also used for a separate analysis. Culture
supernatant samples are automatically transferred to the HPLC information is linked to measurement data files, the C2MAP system
autosampler, where they are dispensed onto a microtiter plate (MTP) can also reduce human error, such as from loading the wrong sample.
for storage. Those samples are automatically supplied for LC/MS/MS Manual methods can cause variations in data quality, but automated
measurement, where 95 components are analyzed simultaneously equipment helps ensure any operator can acquire data with good
using the Cell Culture Profiling Method (equivalent to Ver. 1). After repeatability (Fig. 2). Automation can also reduce operational hours (Fig.
peak integration, time-series data for each component can be 3).
visualized easily (Fig. 1) by loading the data file into the C2MAP

Automatic Pretreatment Autosampler LCMS-8050/-8060 C2MAP TRENDS Purification
System SIL-30AC Cell Culture Profiling
C2MAP-2030 (includes methods equivalent to Ver. 1)

• Organic solvent Characterization
precipitation and • Automatic sample dilution • LC/MS/MS measurement • Visualization of
suction filtration • Dispensing into MTP plates temporal changes
• Sample transferring • Peak integration in each component
Automated process steps
Fig. 1 Analysis Process Flow with C2MAP System

Area Reproducibility for Caffeine Quality Control

Total

Specifications
C2MAP system 70 min Instrument C2MAP-2030
LC unit Nexera X2 (CBM-20A, LC-30AD, CTO-20AC, SIL-30AC, MR 20 μL mixer, and other units)
Conventional MS unit LCMS-8050/-8060
Method (Manual 475 min Required sample quantities 400 to 500 µL (or 100 to 250 µL using optional rack)
Operations) Pharmacokinetics
Processing time 10.8 min per sample (17 min per sample for LC/MS measurements)
C2MAP Operator A Operator B Operator C
Pretreatment processes Add internal standard, sample, and organic solvent, mix, filter by suction filtration, and transfer
Fig. 2 Comparison of Analysis Repeatability Fig. 3 Comparison of Operator Labor Hours sample after processing
Pretreatment methods Batch mode Starts LC/MS measurement after pretreatment is finished for all samples.
Conclusion Sequential mode Each sample processed successively in parallel.
Pretreatment mode All samples are pretreated only.
The C2MAP system can automate the deproteinization process for up Others
to 65 culture supernatant samples. Max. number of samples 65 (or 64 using the sequential mode)
By linking it to an LC-MS system, the entire process from pretreatment External dimensions W 670 × D 700 × H 1,190 mm (C2MAP-2030 only)
to LC/MS/MS measurement can be executed seamlessly.
Weight 185 kg (C2MAP-2030 only)

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