Page 30 - Clinical-Medical Solution for Clinical Chemistry

Application AD-0135
News

 Conclusions  References

A fast MRM-based method for quantitation of five β-lactam 1. Sibhghatulla Shaikh, Jamale Fatima, Shazi Shakil, Syed Mohd.
antibiotics tazobactam, cefepime, meropenem, ceftazidime Danish Rizvi. 2015. 22(1), 90 - 101
and piperacillin in human plasma was developed on LCMS- 2. Mohammad Amjad Kamal. Saudi J Biol Sci. S. K. Grebe, R. J.
8060. A simple sample pre-treatment with protein crash by Singh. Clin. Biochem. Rev. 2011. 32(1), 5 – 31
organic solvent was applied and a small injection volume of 3. Berendsen, B. (2013). LC-MS residue analysis of antibiotics:
2 µL was required due to the high sensitivity of the LCMS- What selectivity is adequate?
8060 employed. The method performance was evaluated 4. Deepti Bhandarkar, Rashi Kochhar, Shailendra Rane, Shruti
Raju, Shailesh Damale, Ajit Datar, Pratap Rasam and Jitendra
on the linearity, accuracy, repeatability, recovery, matrix Kelkar, Highly sensitive quantitative analysis of Amoxicillin and
effect, specificity and limit of quantitation (LOQ). The Clavulanic acid from plaama using LC/MS/MS, ASMS 2015,
estimated LOQs of the method for the five antibiotics are in Poster TP 280 (PO-CON1546E)
the range from 0.7 ng/mL to 6.0 ng/mL with an injection 5. Pieter Colin, Lies De Bock, Huybrecht Tjollyn, Koen Boussery,
volume of 2 µL. and Jan Van Bocxlaer, Talanta 103 (2013) 285-293.
6. Sebastiano Barco, Roberto Bandettini, Angelo Maffia, Gino
Tripodi, Elio Castagnola and Giuliana, J. Chemotherapy Vol 27
(2015) No. 6, 343-347.

Disclaimer: The data and instruments presented in this
Application News are intended for Research Use Only (RUO).
Not for use in diagnostic procedures.

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