Page 10 - Clinical-Medical Solution for Clinical Chemistry

Application No. C153
News

„ Comparative Test with Manual Pretreatment A comparison of quantitative values between the
methods was performed for amiodarone and the
A comparative test was performed between a manual
pretreatment method and the fully automated metabolite desethylamiodarone (Fig. 5 and 6, Table 4
and 5). In the wide range of concentrations detected
pretreatment analysis method that employs the fully
automated sample preparation LC/MS/MS system. from the samples, there was favorable agreement
between the quantitative results of the manual
Human blood plasma for measuring the concentration
of amiodarone was used. pretreatment method and the fully automated
pretreatment analysis method. The coefficient of
The manual pretreatment method involved manually determination (R2) of both methods calculated from
isolating the blood plasma, adding acetonitrile, and these results was 0.95 or higher (Fig. 7 and 8).
mixing to perform deproteinization. After centrifugal The fully automated pretreatment analysis method
separation of this sample, the supernatant was then
transferred to a vial for LC/MS/MS analysis. On the used by this system is a fast and low-burden analysis
technique that achieves quantitative results equivalent
other hand, the fully automated pretreatment analysis
method enabled the entire analysis process, from to conventional manual pretreatment methods and we
anticipate its utilization into the future.
blood plasma isolation to LC/MS/MS analysis, to be
performed completely automatically using the system
described in this article (Fig. 4).

Pretreatment Workflow of the Manual Pretreatment Method and Fully Automated Pretreatment Analysis Method

(x100,000) (x100,000)
1.0 1.0
1:AMD 646.00>58.10(+) CE: -52.0 1:AMD 646.00>58.10(+) CE: -52.0
2:DEA 618.00>72.10(+) CE: -32.0 2:DEA 618.00>72.10(+) CE: -32.0
0.9 0.9
Manual Pretreatment Automated Pretreatment
0.8 0.8
with CLAM-2000
0.7 0.7
Amiodarone Amiodarone
546 ng/mL 557 ng/mL
0.6 0.6
0.5 Desethylamiodarone 0.5 Desethylamiodarone
416 ng/mL 423 ng/mL
0.4 0.4
0.3 0.3

0.2 0.2

0.1 0.1
0.0 0.0

-0.1 -0.1
1.50 1.75 2.00 2.25 min 1.50 1.75 2.00 2.25 min

Human Blood Plasma (Sample 3) Analysis Results Using Human Blood Plasma (Sample 3) Analysis Results Using
the Manual Pretreatment Method the Fully Automated Pretreatment Analysis Method