Page 15 - Shimadzu Journal vol.2 Issue3

Polymer

Fig. 1 shows the SEC chromatogram of poly (MMA-b-n-BMA). The of 142 between adjacent peaks, a distribution indicating the
main peak was collected every 6 seconds from the start to the end existence of the PnBMA homopolymer. On the other hand, the
of elution (8 min 20 sec to 13 min 20 sec) using the AccuSpot, and peaks indicated by in the molecular weight distribution with
50 sample spots were created on the MALDI sample plate. Each m/z 1800 at the top show an interval of 100 between adjacent
spot was measured using the MALDITOFMS instrument, and the peaks, indicating the existence of the PMMA homopolymer. The
mass spectra corresponding to the elution times were obtained above results clearly show that the PnBMA and PMMA
(Fig. 2). The mass spectra obtained from the fractions each show a homopolymers are present as minor constituents in the poly
different molecular weight distribution. Focusing on the fraction (MMA-b-n-BMA) sample used here. The detection of these minor
after 11 min 18 sec, two different types of molecular weight components included in the synthetic polymer demonstrates that
distribution were detected that had not been seen before this SEC-AccuSpot-AXIMA System achieves higher time resolution
separation (Fig. 3). The peaks indicated by in the molecular of eluted constituents than the manual SEC/MALDI-TOFMS
weight distribution with m/z 1200 at the top show an interval technique previously (Fig. 4).

Pre-separation

CH3
(C-CH2) n After 11 min 18 sec
142
COO (CH2)3CH3 CH3
PnBMA homopolymer
(C-CH2)
n
COOCH3
100 PMMA homopolymer

1000 1500 2000 2500 3000 3500
m/z
Fig. 3 Detection of Homopolymer Components

Spotter for MALDI plate
®
Prominence AccuSpot AXIMA Performance

Online

MALDI plate
SEC
Plate sensor
MALDI plate
Before spotting Spotting After spotting

Fig. 4 SEC-MALDI System

NOTES:
This Application News has been produced and edited using information that was available when the data was acquired for each article.
This Application News is subject to revision without prior notice.

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