Page 20 - Shimadzu Journal vol.2 Issue3

Topics

Our paper of monoclonal antibody analysis
by MS selected as Hot Articles

Our paper, Noriko Iwamoto et al., Life Science Research Center, 200 nm nano particles, and detected and quantified by liquid
Shimadzu Corporation, has been selected to the Hot Articles of chromatography/mass spectrometry (LC/MS). This experimental
Analyst in Royal Society of Chemistry. This report is one of the design successfully performed limited proteolysis on
collaboration results of National Cancer Center in Japan. Our new complementarity-determining region of the antibody variable
method, named nSMOL proteolysis (nano-surface and molecular region by limiting protease access to the substrate. This nSMOL
orientation limited proteolysis), is the innovative proposal to the method is made possible therapeutic drug monitoring (TDM)
antibody analysis by mass spectrometry focused on the size of independent of a variety of monoclonal antibodies, and
antibody and its orientation. Immobilized antibody on the resin accelerated the development of TDM system with MS.
with 100 nm pore has been digested by immobilized protease on

Selective detection of complementarity-determining regions of monoclonal antibody by limiting protease access to the substrate:
nano-surface and molecular-orientation limited proteolysis

Noriko Iwamoto, Takashi Shimada, Yukari Umino, Chikage Aoki, Yutaka Aoki, Taka-Aki Sato,
Akinobu Hamada and Hitoshi Nakagama
Analyst, 2014, 139, 576-580
http://blogs.rsc.org/an/2014/02/07/hot-articles-in-analyst-32/
http://pubs.rsc.org/en/Content/ArticleLanding/2014/AN/c3an02104a#!divAbstract

98