Page 8 - Shimadzu Nexera UC

Unified speed of analysis, sensitivity, and resolution

Solutions provided by Nexera UC

Very fast separation speed due to the relatively low viscosity of supercritical fluid

Improved peak capacity and chromatographic resolution
Efficient separation of analogues and/or chiral compounds by high penetration mobile phase

Different separation mode leads to high sensitivity

Improved sensitivity resulting from split-less introduction into detector

Reduction of environmental impacts and costs by reducing amount of organic solvents needed
Sensitivity results from different separation modes in HPLC vs SFC

Supercritical fluid has unique properties different from liquid.
Using SFC in front of a mass spectrometer offers greater sensitivity than achieved with LC/MS/MS.
Higher resolution
LC/MS/MS SFC-MS/MS
6.0 6.0
Improved separation and detection capabilities result from the low viscosity and high diffusion coefficient of supercritical
fluid. As shown below, Nexera UC demonstrates high-separation selectivity for isomeric compounds that are difficult to
separate by conventional LC. 6×
More Sensitive
HPLC Rs = 1.328 1.0
0.0 0.0
0.0 0.5 1.0 1.5 min 0.0 0.5 1.0 1.5 min

Comparison of peak intensity detected by the same MS detector.
(Sample: Prostagrandin D2 10 pg)

Enhanced sensitivity using splitless transfer to MS
0 4 8 12 min
Approx. 1/3 Low dead volume back pressure regulator suppresses diffusion of peaks and can transfer the total eluate directly to a mass
spectrometer to achieve higher sensitivity.

SFC 3.0 3.0
Rs = 1.703 Split Split-less
(Split ratio vent:MS = 8:2) 2.5
2.0 2.0
5×
More Sensitive
1.0 1.0
0.5

0.0 0.0
0 1 2 3 4 min 0.0 0.5 1.0 1.5 min 0.0 0.5 1.0 1.5 min

Comparison of retention time and separation acquired by Conventional LC and SFC Comparison of intensity of two peaks detected by the same MS detector.
(sample: α-tocopherol, column: Shim-pack UC-X Sil) (Sample: Reserpine 10 pg)

Nexera UC
8 Supercritical Fluid Extraction/Chromatograph System 9