Page 5 - Shimadzu Nexera UC

Fully automated on-line sample preparation and

analysis of target compounds

Nexera UC on-line SFE-SFC is a revolutionary system that combines on-line SFE and SFC in a single flow path (patented technology).
Target compounds are extracted from solid samples and then automatically transferred to SFC/ MS so that no human
intervention is required. The Nexera UC on-line SFE-SFC system reduces the time for pretreatment of samples and
acquires highly accurate data.

Comparison of QuEChERS sample preparation and Nexera UC in the analysis of residual pesticides

A typical sample preparation takes 35 minutes and requires several manual steps. With Nexera UC, the same sample can be ready
for on-line SFE/SFC analysis in as little as five minutes with only a few simple sample preparation steps.

QuEChERS ••• Requires 35 minutes of sample preparation 35 min

Homogenize Add solvent Stir Add reagent Stir Centrifuge Transfer Add reagent Stir Centrifuge Transfer
Supernatant Supernatant Analysis with GCMS ™ Analysis with LCMS ™

Hundreds of compounds are simultaneously analyzed with
Nexera UC. These include pesticides that are usually analyzed
SFE with LC and LC/MS/MS or GC and GC/MS/MS. Nexera UC enables
Nexera UC ••• As little as 5 minutes to be ready for analysis 5 min (about 4 minutes) analysis of compounds over a wide polarity range.

0 5 10 15 min
Simultaneous Analysis of 500 Pesticides

1
2 3 Pesticide compound analysis with GC or GC/MS/MS
Enclose in 4
Homogenize Add absorbent* Mix Extraction and analysis with SFE-SFC-MS 5
extraction vessel 6 1. Diazinon 5. Mepronil
7
2. Metalaxyl 6. Dioxathion
3. Tolclofos-methyl 7. Cypermethrin
4. Lenacil
* “Miyazaki Hydro-Protect”, Patented in Japan No. 3645552 8 9
SFE 10 11
(about 4 minutes) 12 13 14 Pesticide compound analysis with LC or LC/MS/MS
15 8. Aramite 12. Cyazofamid
9. Isouron 13. Diquat
10. Acephate 14. Chromafenozide
11. Aminocarb 15. Imidacloprid
0 5 10 15 min
Analysis of Compounds with Wide Polarity Range

Nexera UC
4 Supercritical Fluid Extraction/Chromatograph System 5