Page 4 - Shimadzu Nexera UC

Fully automated on-line sample preparation and

analysis of target compounds

Nexera UC on-line SFE-SFC is a revolutionary system that combines on-line SFE and SFC in a single flow path (patented technology).
Target compounds are extracted from solid samples and then automatically transferred to SFC/ MS so that no human
intervention is required. The Nexera UC on-line SFE-SFC system reduces the time for pretreatment of samples and
acquires highly accurate data.

Comparison of QuEChERS sample preparation and Nexera UC in the analysis of residual pesticides

A typical sample preparation takes 35 minutes and requires several manual steps. With Nexera UC, the same sample can be ready
for on-line SFE/SFC analysis in as little as five minutes with only a few simple sample preparation steps.

QuEChERS •• • Requires 35 minutes of sample preparation 35 min

Homogenize Add solvent Stir Add reagent Stir Centrifuge Transfer Add reagent Stir Centrifuge Transfer
Supernatant Supernatant Analysis with GCMS ™ Analysis with LCMS ™

Hundreds of compounds are simultaneously analyzed with
Nexera UC. These include pesticides that are usually analyzed
SFE with LC and LC/MS/MS or GC and GC/MS/MS. Nexera UC enables
Nexera UC •• • As little as 5 minutes to be ready for analysis 5 min (about 4 minutes) analysis of compounds over a wide polarity range.

0 5 10 15 min
Simultaneous Analysis of 500 Pesticides

1
2 3 Pesticide compound analysis with GC or GC/MS/MS
Enclose in 4
Homogenize Add absorbent* Mix Extraction and analysis with SFE-SFC-MS 5
extraction vessel 6 1. Diazinon 5. Mepronil
7
2. Metalaxyl 6. Dioxathion
3. Tolclofos-methyl 7. Cypermethrin
4. Lenacil
* “Miyazaki Hydro-Protect”, Patented in Japan No. 3645552 8 9
SFE 10 11
(about 4 minutes) 12 13 14 Pesticide compound analysis with LC or LC/MS/MS
15 8. Aramite 12. Cyazofamid
9. Isouron 13. Diquat
10. Acephate 14. Chromafenozide
11. Aminocarb 15. Imidacloprid
0 5 10 15 min
Analysis of Compounds with Wide Polarity Range

Nexera UC
4 Supercritical Fluid Extraction/Chromatograph System 5