Page 12 - Shimadzu Nexera UC

System configuration examples

On-line SFE-SFC-MS System Nexera UC/s SFC/UHPLC Switching System

In this system, solid samples are extracted by supercritical fluid and introduced to SFC on-line. The time This system can switch automatically between SFC analysis and UHPLC analysis and make measurements
for pretreatment of samples is drastically shortened. In addition, samples are extracted under on a single sample in each separation mode. It enhances user-friendliness and operability by allowing the
light-shielding and anaerobic conditions in order to protect labile analytes from degradation. investigation of separation conditions and performing reverse-phase high-speed analysis in a single
system. Shimadzu also provides a kit to upgrade from your current UHPLC system to the UHPLC/SFC
switching system.

Simultaneous analysis of pesticides over a wide range of polarity Analytical Fraction System
With conventional methods, both LC (LC/MS/MS) and GC (GC/MS/MS) are needed to analyze pesticides. In contrast,
the Nexera UC On-line SFE-SFC system can extract and analyze the full range of pesticides automatically.
Pretreatment requires only homogenization and dehydration, which takes 1/7 the time of the QuEChERS method. By adding the FRC-40 SF fraction collector to the Nexera UC, small-volume fractionation is also possible. A
Adding the optional Rack Changer enables automated analysis of up to 48 samples. complete workflow can be carried out seamlessly on one system, from evaluation of analytical conditions
with method scouting to fractionation on the order of a few mg. Shimadzu’s unique, patented
Extraction and analysis of labile samples without degradation LotusStream separator successfully reduces sample dispersion and carryover while also achieving high

Samples are extracted under light-shielding and anaerobic conditions so that labile compounds can be analyzed recovery rates.
without degradation.

Quick start of cleaning validation in pharmaceutical industry

Regardless of the sample polarity, the only required preparation step is enclosing the sample swab in an extraction
vessel. The Nexera UC On-line SFE-SFC-MS system automatically starts extraction for analysis.

Analysis of polymer additives where other analytical instruments struggle

Polymer additives are insoluble in THF or chloroform and hard to sublime; therefore, GPC and GC are not
applicable. The Nexera UC On-line SFE-SFC system easily extracts and analyzes them.

Nexera UC
12 Supercritical Fluid Extraction/Chromatograph System 13