Page 5 - Shimadzu Co-Sense Series

Analyzing Biological Samples Using Co-Sense for BA

A major factor when analyzing low molecular weight compounds in biological samples, such as blood plasma and blood serum, is
sample pretreatment, which mainly time-consuming deproteinization by liquid-liquid or solid phase extraction. However, by using
the Shim-pack MAYI series on-line pretreatment column in combination with Co-Sense for BA, automated pretreatment and
quantification of target compounds can be achieved in a fraction of the time compared to traditional methods. The system
automation provides a high degree of reproducibility and confidence.

Shim-pack MAYI Series
Proteins and other
macromolecules
The Shim-pack MAYI series are restricted access media columns.
Drug
They are on-line pretreatment columns for biological samples
Mobile phase
that feature a hydrophilic surface coating and optimized
Coating layer
particle sizes to achieve highly efficient deproteinization and
long-term stability. This provides excellent repeatability even for
continuous analysis of multiple samples. In addition, five kinds
of solid phases are offered for traps based on hydrophobic
interaction (C1, C4, C8, C14, and ODS), and strong cation Mobile phase
exchange (SCX) and strong anion exchange (SAX) mode solid
phases are offered for traps based on ion-exchange effects. Illustration of Shim-pack MAYI Series

1
Superior Recovery Rate Due to Dilution Trap About 100 %
recovery
1
1. Indomethacin
The concentration trap column in Co-Sense for BA inhibits the
target components from interacting with proteins in samples. 1
About 50 %
This allows reliably trapping and concentrating even recovery
components with high protein binding rates. A buffer solution
With dilution
or a solution containing a low-concentration organic solvent is
Without dilution
used as the dilution solvent. As a result, even blood plasma (or Standard solution
blood serum) samples can be injected directly. In addition, due
0 4 8 12 16
to excellent recovery rates and superior peak shapes, high Time (min)
sensitivity is achieved even for large-volume injections. Effect of Dilution Trap on Analysis of Indomethacin in Blood Plasma

Example of Analyzing Drugs in Blood Plasma (×10,000)
281.00 (1.00)
9.0 256.00 (1.00)
Higher efficiency and sensitivity can be achieved by using LC/MS 414.00 (1.00) 2
8.0 260.00 (1.00) 4
for detection in the Co-Sense for BA system. In addition to 455.00 (1.00)
7.0 1
saving time and money normally required for pretreatment by 1. Noscapine
6.0 2. Propranolol 3
injecting blood plasma directly, LC/MS also allows quicker 3. Diphenhydramine
5.0 4. Imipramine
detection of specific components. Furthermore, automatic 5. Verapamil
pretreatment by Co-Sense for BA inhibits matrix effects and 4.0 5
allows higher precision analysis, even for direct injection of 3.0
blood plasma. 2.0
1.0
0.0
3.0 4.0 5.0 6.0 min
SIM Chromatogram of Five Basic Drug Components in Blood Plasma
(50 µL of blood plasma was directly injected.)

Co-Sense Series
High Performance Liquid Chromatograph 5