Page 10 - Shimadzu Co-Sense Series

Analyzing Fat-Soluble Components Using Co-Sense for Impurities

The dilution trap in Co-Sense for Impurities allows combining various separation modes. Therefore, it can be used to automate first
dimension pretreatment processes that previously served as an off-line pretreatment role and convert a separation mode not
suitable for LC/MS to one optimal for LC/MS analysis.

Example of Using LC/MS to Analyze Vitamin D 3 in a Vitamin Supplement

When analyzing fat-soluble components, samples are often pretreated using solid phase extraction to remove fat-soluble matrices.
This can be automated using Co-Sense for Impurities with the first dimension for on-line pretreatment. It can also be used for
combining normal and reversed phase separation modes.

Example of Analyzing Vitamin D 3 in a Vitamin Supplement
<First Dimension>
Matrix removal by
normal phase separation The vitamin D 3 was separated from the matrix inside a soft capsule by
Vitamin D 3
(Trap) using the normal phase mode for first dimension separation. After
dilution trapping, the vitamin D 3 in the vitamin supplement can be
analyzed without matrix effects by delivering it to LC/MS using reversed
phase separation.
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<Second Dimension>
LC/MS analysis of vitamin D 3
by reversed phase separation
Vitamin D3

0.0 2.5 5.0 min

Example of Analyzing Phospholipids by LC/MS
With phospholipid analysis using only reversed phase separation, it is difficult to quantify the phospholipids appropriately, due to
ion suppression or enhancement effects resulting from various co-existing phospholipids. However, the adoption of Co-Sense for
Impurities allows quantifying phospholipids precisely by using normal phase separation to separate phospholipids by form in the
first dimension and then using reversed phase separation in the second dimension to deliver samples to LC/MS.

Example of Analyzing Phospholipids
<First Dimension> PC
Normal phase separation by form
In the first dimension, phosphatidylserine (PS) is separated from other
phospholipids by normal phase separation. After dilution trapping, the
PG
PE samples are separated based on fatty acids in the reversed phase
PI
PS separation and then detected by LC/MS. This inhibits any impacts from
0 5 10 15 20 25 30 35 min other phospholipids and allows analyzing only phosphatidylserine (PS).
Trapping PS Fraction

<Second Dimension>
LC/MS analysis of PS
by reversed phase separation

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