Page 11 - Shimadzu Co-Sense Series

Analyzing Natural Products Using Co-Sense for Impurities

Analyzing ultra-trace components in natural products requires both reliable separation of target components from contaminant
components and increased sensitivity. In particular, concentrating liquid samples, such as fruit juices, in the pretreatment process not
only reduces throughput, but can also cause cross contamination. However, Co-Sense for Impurities is able to analyze such liquid
samples efficiently by direct injection. Furthermore, using an ultra-fast separation column can significantly reduce the separation time.

Analysis Using a Traditional System

When analyzing trace components in natural products with a traditional system, it is difficult to achieve both the separation and
high sensitivity necessary to eliminate the impacts of contaminants and selectively detect target components.

UV 320 nm

Ferulic acid
Standard
sample
Actual
sample
0.0 1.0 2.0 3.0 4.0 5.0 min 250 300 350 nm
Chromatogram Peak Spectrum of Ferulic Acid

Example of Using a Traditional System for Analyzing Berry Juice
A comparison of the absorbance spectra for ferulic acid in standard and actual samples does not show adequate matching, which suggests the spectra
were affected by contaminant components.

High-Sensitivity Analysis Using Co-Sense for Impurities

By using the Shim-pack XR-ODS ultra-fast separation column for both the first and second dimensions and by changing the mobile
phase pH level used for separation in the first and second dimensions, both fast separation and high sensitivity were achieved by
reliably separating contaminant components.

<First Dimension> <Second Dimension>
Phosphate buffer solution Acetate buffer solution with pH 4.7
with pH 2.5
Ferulic acid

Ferulic acid
(trap) Standard sample
Actual sample

0.0 0.5 1.0 1.5 min 0.0 2.0 4.0 min 250 300 nm
First Dimension Chromatogram Second Dimension Chromatogram Second Dimension Peak Spectrum of Ferulic Acid

Example of Analyzing Ferulic Acid in Berry Juice
With reliable separation of contaminant components by automated on-line pretreatment (absorption spectra of standard and actual samples matched)
and high sensitivity, good precision was achieved even for the actual sample, with a peak area repeatability of 0.6 % RSD. Also, using the Shim-pack
XR-ODS ultra-fast separation column allowed achieving high-speed two-dimensional separation.

Co-Sense Series
High Performance Liquid Chromatograph 11