Page 176 - Shimadu Consumables Catalog

GC Columns

Capillary Columns
General-Purpose Columns

SH-2560

Highly polar phase; biscyanopropyl polysiloxane—not bonded
Stationary phase selectivity optimized for isomer separation to ensure accurate quantification of critical cis/trans FAMEs.
Application-specific QC test guarantees consistent, reliable performance for AOAC 996.06 and AOCS Ce 1j-07 methods.

Excellent sample capacity; no peak distortion means easy, accurate peak integration.
Similar Phase(s): HP-88, CP-Sil 88, SP-2560, BPX-90, MEGA-10

ID df Temp. Range 100 m
0.25 mm 0.20 µm 20 to 250 °C 227-36311-01

Unsaturated FAMES QC Test Mix on SH-2560

Conc. Structural Conditions
Peaks tR (min) (µg/mL) Nomenclature Column : SH-2560, 2560, 100 m, 0.25mm
1. Methyl octadecanoate 11.68 1.1 C18:0 ID, 0.20 μm (227-36311-01)
2. Methyl elaidate 12.64 0.8 C18:1 (t9) Sample : Unsaturated FAMES QC test mix
3. Methyl oleate 13.09 0.6 C18:1 (c9)
4. Methyl nonadecanoate 13.84 1.1 C19:0 Diluent : Hexane
5. Methyl eicosanoate 16.66 1.1 C20:0 Injection : 1 µL split (split ratio 200:1)
6. Methyl eicosenoate 18.85 0.6 C20:1 (c11) Liner : Premium 4 mm Precision liner
7. Methyl linolenate 19.20 0.8 C18:3 (c9,c12,c15) w/wool (cat.# 23305.1)
8. Methyl heneicosanoate 20.35 1.1 C21:0 Inj. Temp : 250 °C
9. Methyl docosanoate 25.17 1.1 C22:0 Oven Temp : 180 °C (hold 40 min)
10. Methyl eicosatrienoate 28.79 0.6 C20:3 (c11,c14,c17)
11. Methyl tricosanoate 31.49 1.1 C23:0 Carrier Gas : H , constant flow
2
Flow Rate : 2.5 mL/min
Detector : FID, 275 °C
Make-up Gas Type : N 2
Hydrogen flow : 40 mL/min
Air flow : 400 mL/min
1 Data Rate : 50 Hz
Instrument : Agilent 7890B GC
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2 5
8
3
7 9
11
6
10

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Time (min)

Pg C-26