Page 8 - Shimadzu-FoodSafety

Analysis of Mycotoxins Identification and Observation of Microorganisms

Mycotoxins are chemical products produced in fungus and are toxic to humans, animals, and crops. There are more than 300 In the event of food poisoning, identification of the causative factor is extremely important in determining the path of
known mycotoxins, including aflatoxin, deoxynivalenol, patulin, fumonisin and others, and some of them are strictly infection, prescribing treatment and preventing recurrence. Shimadzu’s iDPlus microorganism identification platform provides
controlled under regulations. HPLC and LC-MS are mainly used for analysis of mycotoxins, and simultaneous analysis of rapid identification of microorganisms in food.
multiple mycotoxins has been carried out for more efficient screening.
Identification of Microorganisms (MALDI-TOF-MS) AXIMA Microorganism Identification System
In order to prevent pathogenic germs from contaminating
Analysis of Aflatoxins (LC) Rapid Screening of 10 Mycotoxins (LC)
food sources, it is essential to identify them quickly and
The analysis of aflatoxins (B1, B2, G1 and G2) is very Simultaneous analysis of mycotoxins is important because reliably in order to allow for proper controlled measures.
important from a food safety perspective due to concerns multiple mycotoxins have been detected in a single food and A microorganism identification system using the AXIMA
about adverse effects on human health. The Nexera Ultra may cause a synergistic effect. From this background, a MALDI-TOF MS enables direct measurement of cultured
High Performance Liquid Chromatograph achieves simultaneous analytical method has been developed for microorganisms and identification within two minutes.
high-resolution analysis of aflatoxins in less than four rapid screening of 10 mycotoxins using HPLC. Shown below
% Int.
minutes. The RF-20Axs high-sensitivity fluorescence detector are chromatograms of 10 mycotoxins by fluorescence (upper) Salmonella enterica subsp. Enterica
allows direct analysis of aflatoxins at trace levels (ppt) and PDA (lower) detectors without derivatization. 4365.39 5369.13 6091.59
without any derivatization. 10950.18
mV 7158.16 7658.66
3 3829.04
Integrated HPLC RF-20AXS 4 ■Peaks 9520.27
Peaks Nexera-i 2 1) AFM1 1 µg/L 6885.93
2) AFG2 0.25 µg/L
1 AFG2 (0.6 µg/kg) 2 3) AFG1 0.25 µg/L
2 AFG1 (2.4 µg/kg) 1 4) AFB2 0.25 µg/L
3 AFB2 (0.6 µg/kg) 5) AFB1 0.25 µg/L 5033.17 5524.87 Staphylococcus aureus subsp. Aureus
4 AFB1 (2.4 µg/kg) 6) ZON 50 µg/L 4308.68
1 7) OTA 5 µg/L 6352.79
7 12397.15 13762.64
5 3640.52
0 3 6
4 5 6 7 8 9 10 11 4434.57 5209.27 6043.95 6671.91 7230.02 8348.89 Pseudomonas aeruginosa
min 7886.39 11424.07
mAU 4054.41 7590.49 10220.70 11027.94 11922.76 12295.04 12706.04 13225.69 13698.41 14067.89 14991.82
Nexera-i PDA ■Peaks
5 8) NIV 1000 µg/L
8 9) DON 1000 µg/L 7156.44
10) PAT 25 µg/L 6253.14 9059.12 9733.78 Escherichia coli
9 7867.55 Features
Analysis of Aflatoxins without TFA Derivatization （N = 5） 8364.74
0 220 nm 8871.39
10 13645.06 Quick and Easy Analysis
Source: Shimadzu Application News No. L435 276 nm
0.0 0.5 1.0 1.5 2.0 2.5 3.0 Bacillus subtilis Three steps from sample preparation to identification.
min 4307.86 5255.03 9235.80 The microorganism identification process is finished within two
Simultaneous Analysis of 10 Mycotoxins 3890.60
Simultaneous Analysis of 25 Mycotoxins (LC-MS/MS) Source: Shimadzu Application News No. L512 minutes after starting the analysis.
4000 6000 8000 10000 12000 14000 Highly Accurate Identification
LC-MS/MS is effective for analysis of mycotoxins in complex m/z Microorganism identification by MALDI-TOF MS is a well-established
matrices. Shown is an example of the simultaneous analysis technique with a low number of false positives.
Comparing mass spectra of different bacteria
of 25 mycotoxins by LC-MS/MS and the Method Package for Database Source: https://www.shimadzu.com/an/industry/pharmaceuticallifescience/proteome0207005.htm Applicable for a Wide Range of Microorganisms
Mycotoxins. All compounds, including positive and negative Capable of classifying/identifying gram-positive and gram-negative
ions, were separated and detected with high sensitivity LC/MS/MS Method Package for Mycotoxins What is Microorganism Identification by MALDI-TOF MS? bacteria, yeasts, fungi and spores. iDPlus identifies mircoorganisms
The Method Package provides simultaneous multi-component analysis by family, genus, species, and subspecies.
within 12 minutes. For analysis of mycotoxins with high
conditions for mycotoxins subject to regulations in the US, the EU, Species-specific mass spectral patterns from microorganisms can be Database Customization
sensitivity, eliminating carryover is indispensable. Modified
China and Japan. It contains seven different analytical method obtained by direct measurement of microorganisms using MALDI-TOF Easily register new microorganisms in the database, which allows
rinse methods using the SIL-30AC autosampler can eliminate
conditions and fully optimized MRM transitions for a total of 27 MS. Identification of microorganisms can be performed by matching rapid development of a high-quality database.
carryover of fumonisins.
different mycotoxins. Rapid analytical methods are included for a 25 these collected spectra to the database. This is a fast, easy and Low Operational Cost
mycotoxin screen, 16 mycotoxins using high-sensitivity conditions, 10 reliable method for identification of microorganisms. Analysis can be performed for pennies per sample compared to
mycotoxins using APCI, and specialized methods for trichothecenes, expensive traditional techniques.
fumonisins, aflatoxins, and patulin. Expanded Research Capabilities
iDPlus is not limited to microorganism identification. Reflectron
Number of Analysis mode and MS/MS functionalities enable a true research platform.
Method
Components Time (min)
Simultaneous Analysis of 25 Mycotoxins 25 15
Simultaneous Analysis with High Sensitivity 16 30
Patulin Analysis 1 10
Aflatoxins Analysis 6 30
Trichothecenes Analysis 4 12
High-speed Simultaneous Analysis of 25 Mycotoxins Fumonisins Analysis 3 15
Source: Shimadzu Flyer No. C146-E351 10 mycotoxins using APCI 10 33
Shimadzu’s Total Support for Food Safety
8 Analytical and Testing Instruments for Food Safety 9