Page 10 - Shimadzu-FoodSafety

Analysis of Toxic Metals Analysis of Food Additives

Toxic metals (arsenic, lead, cadmium, etc.) in foods must be controlled down to low concentration levels. A wide variety of additives, such as preservatives, flavor enhancers, coloring agents and antioxidants, are used on foods.
Shimadzu atomic absorption spectrophotometers and ICP emission spectrometers offer highly sensitive analysis of trace Strict monitoring of food stuffs is essential to detect the use of prohibited chemicals. Each country assigns a unique number or
elements in foods. Shimadzu’s product lineup also includes X-ray fluorescence spectrometers that can perform elemental code of approved chemicals for that country. Shimadzu offers a wide range of products for detecting the prohibited
analysis without sample pretreatment. These instruments are ideal for screening analysis and emergency analysis. chemicals, whether they were added intentionally or through contamination.

Analysis of Cd and Pb in Food Additives (AA) Atomic Absorption Spectrophotometer AA-7000 Analysis of Dicyandiamide and Melamine in Milk Powders (LC-MS/MS)
Shown here is an analysis of cadmium (Cd) and lead (Pb) in Melamine was found to be used as a protein-rich adulterant residues were found in milk products from New Zealand.
α-cyclodextrin (cyclic oligosaccharide), a substance used in in pet food in 2007, and then in infant formula in 2008 in Instead of being added directly as an adulterant, the trace
functional foods, pharmaceuticals, cosmetics, etc. The China. The outbreak of the melamine scandal that killed DCD, which is used for promote the growth of cow pastures,
analysis was conducted by the AA-7000 atomic absorption many dogs and cats as well as led to the deaths of six infants found in milk products was explained to be relating to the

Abs Abs and illness of many others caused panic and great concerns grass “contaminated by DCD”.
Cd Pb worldwide. Recently, low levels of dicyandiamide (DCD) (x100) (x1,000)
STD 1 µg/L 6.0 4.5
Area (x10,000) Area (x100,000) DCD 4.0 Melamine
STD 10 µg/L 3.5 5.0 (85.1>68.1) 3.5 (127.1>85.1)
7.5 DCD (85.1>68.1) Melamine (127.1>85.1)
R2 = 0.997 3.0 R2 = 0.999 4.0 3.0
Sample
Sample Sample 2.5 2.5
Sample
w/Spike
w/Spike
w/Spike
w/Spike 5.0 2.0 3.0 2.0
Measurement Results of Cd and Pb in α-cyclodextrin
1.5 2.0 1.5
Sample
Sample Element Cd Pb 2.5 1.0 1.0
Sample w/o Spike 1.0
Sample
w/o Spike
w/o Spike
w/o Spike Measured value <0.003 µg/g <0.07 µg/g 0.5 0.5
0.0 0.0
Spike and recovery rate 105 % 99 % 0.0 0.0
0 3sec 0 3sec 0.0 2.5 5.0 7.5 Conc. 0.0 2.5 5.0 7.5 Conc. 2.00 2.25 2.50 2.75 min 5.5 6.0 6.5 min
Peak Profiles of Cd and Pb Calibration Curves of DCD and Melamine Overlapping of Six MRM Peaks of 0.5 ng/mL DCD and Melamine in Milk
Powder Matrix
Analysis of Trace Element Contamination in a Fish ICP Atomic Emission Spectrometer ICPE-9800 Series Analysis of Preservatives in Food (UV) Analysis of Sudan Dyes in Curry Powder (LC)
Sample (ICP)
Below are examples of the analysis of food samples spiked with Sudan dyes are oil-soluble synthetic dyes used in industrial
Fish Protein Certified Reference Material for Trace Metals preservatives. products. Their use is not authorized in food products in some
(DORM-4) was used as the fish sample. The Shimadzu
ICPE-9800 can provide a rapid method to simultaneously 0.7 countries. This is an example of LC analysis of Sudan dyes in
8µg curry powder.
analyze trace elements in fish. The results show excellent
0.6
correlation with the certified reference material. 6µg
0.7 0.5µg 0.5 mAU 3 4 Peaks
0.6 1 Sudan Orange G-a
Cd 214.438nm Standards Cr 205.552nm Standards 0.5 0.4µg Abs. 0.4 4µg 2 Sudan Orange G-b
3 Sudan Red G
Abs. 0.4 0.3µg Sample 0.3 20 5 6 4 Sudan I
Samples Samples 0.3 0.2µg 0.2 2µg 15 5 Sudan II
6 Sudan III
0.2 2 8 7 Sudan Red 7B
Peak Profiles of Cd and Cr 0.1 0.1µg 0.1 7 8 Sudan IV
10 Curry powder
450 500 550 600 650 250 nm 300 350 1 +Sudan dyes
nm
5
Usage and Features of Elemental Analysis System Sodium Nitrite in Meat Sorbic Acid in a Processed
White Fish Product Curry powder
Product Usage / Feature Sample 0
0.0 2.5 5.0 7.5 10.0 12.5 15.0 min
Atomic absorption spectrophotometry is widely used for tests compliant with various standards UV-VIS Spectrophotometer UV-1900
Atomic Absorption Sudan Dyes in Curry Powder
Spectrophotometer due to its high sensitivity. AA-7000 offers superior expandability due to its flexible system Liquid (or sample
configuration. It also offers numerous safety features, such as a vibration sensor and a gas
dissolved in solvent)
AA-7000 Series Features Source: Shimadzu Analysis Guidebook No. C180-E059, page 77
leakage detection system, as standard.
High Performance
ICP atomic emission spectrometry is widely used for tests compliant with various standards due to
ICP Atomic Emission Liquid (or sample ・Ultra-fast scan speed (29,000nm/min)
Spectrometer its high sensitivity and rapidity when performing multi-element simultaneous analyses. ICPE-9800 dissolved in solvent)
series offers numerous features, including the simultaneous analysis of all elements, simple
ICPE-9800 Series ・Low stray light (less than 0.5% at 198nm)
operation with its assistant functions, and low running costs.
・High photometric repeatability (less than
ICP mass spectrometry is a method of analysis that provides ultra-high sensitivity at ppt levels in ±0.0002Abs at 0.5Abs and 1.0Abs)
ICP Mass Spectrometer addition to simultaneous multi-element analysis capabilities. ICPMS-2030, while being highly Liquid (or sample Intuitive Operability
ICPMS-2030 sensitive, also offers low running costs by reducing the argon gas flow. It also greatly improves dissolved in solvent)
the reliability of data through its assistant functions. Easy-to-navigate color touch-screen display
Instrument Validation
X-ray fluorescence spectrometry makes possible the nondestructive analysis, without
X-Ray Fluorescence pretreatment, of the elements in solid, powder, or liquid samples. EDX-7000/8000 are capable of Automatic/semi-automatic validation of
Spectrometer Solid, powder, liquid
EDX-7000/8000 detecting sub-ppm level heavy elements. Offering low running costs and superior maintenance wavelength accuracy and wavelength
features, these instruments are suitable for the screening of toxic metals. reproducibility.
Shimadzu’s Total Support for Food Safety
10 Analytical and Testing Instruments for Food Safety 11