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Analysis of Residual Pesticides                                                                                           Analysis of Veterinary Pharmaceuticals






                    Gas Chromatograph (GC)                             Liquid Chromatograph (LC)                                              Veterinary pharmaceuticals are used in the diagnosis, cure, mitigation, treatment, and prevention of diseases in animals as
                                                                                                                                              well as in the promotion of growth. However, there is increasing concern about these drugs entering the food chain and their
                    Capillary GC can be utilized for the analysis of residual   GC or GC-MS techniques may not be suitable for analysis of
                    pesticides in foods. Using a selective detector such as FPD,   residual pesticides that thermally decompose or have a     effects on the human body. These drugs are categorized in many classes, such as anabolic hormones, anthelmintics,
                    FTD or ECD offers highly sensitive analysis of trace pesticides.  highly polar chemical structure. In contrast, an HPLC   antibiotics, beta-agonists, coccidiostats, corticosteroids, nonsteroidal anti-inflammatory drugs (NSAIDs), and sedatives. Below
                                                                       equipped with a photodiode array detector can acquire                  are a few examples of veterinary pharmaceutical analyses.
                                                                       reliable chromatograms with spectral information for
                             methamidophos  chlorpyrifos-methyl + dimethoate  (E)-dimethylvinphos  fenthion  (Z)-dimethylvinphos  (Z)-chlorfenvinphos  fosthiazate 2 + prothiofos  building libraries to compare compounds. The Shimadzu   Analysis of Multiple Veterinary Pharmaceuticals in Pork (LC-MS/MS)
                                                                       carbamate analysis system incorporates derivatization

                           dichlorvos  acephate  thiometon  salithion  terbufos  diazinon etrimfos iprobenfos  dichlofenthion  cyanophos tolclofos-methyl pirimiphos-methyl  formothion  malathion fenitrothion (MEP)  isophenphos + quinalphos  phenthoate  profenofos + methidathion  ethion + sulprofos  pyridaphenthion  technologies to achieve high-sensitivity, selective analysis of   Veterinary pharmaceuticals are often used to promote the growth of farm animals, but many of these compounds are subject
                                                                       N-methyl carbamate pesticides.
                                                                                                                                              to residue standards. From this background, there is a need for a highly sensitive and rapid analytical technique to analyze as
                                  cadusafos ethoprophos  phorate  chlorpyrifos  (E)-chlorfenvinphos  fosthiazate  propaphos  butamifos  edifenphos  fensulfothion  cyanofenphos  EPN  phosmet  phosalone  pyraclofos  60 mV  many of these compounds as possible in a single run. LC-MS/MS enables simultaneous analysis of multiple veterinary
                                                                                                                                              pharmaceuticals with high sensitivity.
                                                                             50      Oxamyl  Methomyl  Methiocarb sulfoxide  Aldicarb
                                     Pesticide-spiked meat dumpling sample (0.2 µ g/g equivalent)  Methiocarb sulfone  Carbaryl                                                                                    Triple Quadrupole Mass Spectrometer
                                                                             40                       Ethiofencarb
                                                    Normal meat dumpling sample                    Bendiocarb  Fenobucarb                                                                                          LCMS-8060
                                                                             30                                 Methiocarb
                       Chromatograms of Normal Meat Sample and Pesticides-spiked Meat Sample
                                                                             20
                     Gas Chromatograph Nexis GC-2030                         10
                                                                             0
                                                                              0.0   5.0   10.0   15.0  20.0   25.0 min
                                                                          Chromatogram of a Standard Mixture of 10 N-Methylcarbamate Pesticides
                                                                                                                                                            MRM Chromatograms of 89 Veterinary Pharmaceuticals in Pork
                                                                        High-Performance Liquid Chromatograph                                                                        Source: Shimadzu Application News No. C99
                                                                        Prominence Series
                                                                        Carbamates Pesticides Analysis System
                                                                                                                                              Analysis of Veterinary Pharmaceuticals Using MRM Spectrum Mode (LC-MS/MS)
                                                                                                                                              MRM Spectrum Mode was developed to reduce false positives and false negatives in analysis of veterinary pharmaceuticals.
                                                                                                                                              MRM Spectrum Mode acquires a high number of fragment ion transitions for each target compound and generates
                                                                                                                                              fragmentation spectra. In examination of quantifying and identifying 212 veterinary pharmaceuticals, the level of confidence
                                                                                                                                              in compound identification and verification increased in MRM Spectrum Mode, and limits of detection, linearity or
                    Features
                                                                                                                                              repeatability were not compromised compared to a conventional method (2 MRM / compound).
                    Easy Operation
                     An advanced interface enables intuitive operation with clear
                     graphics. Shimadzu's latest tool-free maintenance technology makes
                     daily maintenance easy.
                    High Sensitivity and Reproducibility               Features
                     Achieves high sensitivity with all of the detectors, such as FID and   Rapid Injection
                     BID. The advanced flow controller (AFC) enhances reliability with   An injection time of 10 seconds for a 10 µL sample enhances analysis
                     excellent repeatability.                           efficiency.
                    Exceptional Expandability and Productivity         High Sensitivity and Linearity over a Broad Range
                     Nexis GC-2030 can be customized to meet customers' specific   Upgraded optical and electrical systems reduce noise for lower
                     requirements and needs. Options and functions to use hydrogen   detection limits and ensure simultaneous analysis of major
                     carrier gas safely in high-speed analysis maximize analysis   components and trace components.
                     productivity.                                     Low Sample Carryover
                                                                        Platinum coating minimizes the adsorption of sample components
                                                                        on to the needle surface.                                                                         MRM Spectra and Calibration Curve of Sulfamerazine (1 pg/µL to 100 pg/µL)
                                                                                                                                                                                                                       Source: Shimadzu Application News No. C161




                                                                                                                                                                                          Shimadzu’s Total Support for Food Safety
             6                                                                                                                                                                                                              Analytical and Testing Instruments for Food Safety  7
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