Page 13 - Shimadzu Journal vol.7 Issue2

Food Development

1-4. Conclusion Kimura, T., Hamase, K., Miyoshi, Y., Yamamoto, R., Yasuda, K., Mita, M.,
Rakugi, H., Hayashi, T., Isaka, Y.: Chiral amino acid metabolomics for
In this study, we developed an LC-MS/MS analytical method for high-sensitivity novel biomarker screening in the prognosis of chronic kidney disease. Sci.
quantitative analysis of trace amounts of D-amino acids. Rep., 6, 26137 Available at:
First, the MRM transitions of MS/MS were optimized, then various http://www.ncbi.nlm.nih.gov/pubmed/27188851 [Accessed November
parameters were investigated in detail including interface probe 28, 2016].(2016).
position, temperature, gas flowrate, and voltage, then the sensitivity of Konya, Y., Bamba, T., Fukusaki, E.: Extra-facile chiral separation of amino
target amino acid detection was improved. We encountered an issue acid enantiomers by LC-TOFMS analysis. J. Biosci. Bioeng., 121, 349–353
during separation in which some amino acids were coeluted preventing Available at:
recognition of their individual peaks, but using a column with a https://www.sciencedirect.com/science/article/pii/S1389172315002662
complementary structure alongside the original column allowed analysis [Accessed October 9, 2018].(2016).
of all target amino acids. Next, we verified the measurement range, Konya, Y., Taniguchi, M., Fukusaki, E.: Novel high-throughput and
linearity, repeatability, limit of detection, and lower limit of quantitation widely-targeted liquid chromatography–time of flight mass spectrometry
of the newly developed method, and evaluated its performance in method for d-amino acids in foods. J. Biosci. Bioeng., 123, 126–133
quantitative analysis of trace amounts of D-amino acids. We also (2017).
performed quantitative analysis of a sample (black vinegar) for D-amino Konya, Y., Taniguchi, M., Furuno, M., Nakano, Y., Tanaka, N., Fukusaki,
acids using the newly developed method. Comparing results with an E.: Mechanistic study on the high-selectivity enantioseparation of amino
existing LC-TOFMS method revealed a very similar D-amino acid profile, acids using a chiral crown ether-bonded stationary phase and acidic,
and also revealed that some D-amino acids were only quantified by the highly organic mobile phase by liquid chromatography/time-of-flight mass
LC-MS/MS method, demonstrating a significant advantage of this spectrometry. J. Chromatogr. A, 1578, 35–44 Available at:
https://www.sciencedirect.com/science/article/pii/S0021967318312494
method in terms of detection sensitivity.
[Accessed October 29, 2018].(2018).
Although a number of past reports have used LC-MS/MS to analyze Miyoshi, Y., Nagano, M., Ishigo, S., Ito, Y., Hashiguchi, K., Hishida, N.,
trace amounts of D-amino acids, a distinguishing feature of the LC-MS/MS Mita, M., Lindner, W., Hamase, K.: Chiral amino acid analysis of Japanese
method we describe is that it requires no derivatization and is capable traditional Kurozu and the developmental changes during earthenware jar
of high-throughput and high-sensitivity simultaneous analysis of chiral fermentation processes. J. Chromatogr. B. Analyt. Technol. Biomed. Life
amino acids. The ability to process many samples in a short period of Sci., 966, 187–92 Available at:
time represents a major advantage for this analytical method. http://www.sciencedirect.com/science/article/pii/S1570023214000464
[Accessed February 19, 2016].(2014).
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