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Glycan Analysis
929 931 933 1337 1339
1659 1661 1663 2269 2271 2273
Indicate one of oxidized carbohydrate
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Fig. 5 The list of identified structures of glycans and their oxidized products using MS detection of oxidized glycans
Summary
• The mild oxidation condition was able to specifically oxidize sialic acid group at the end of carbohydrate chain. Thus, the sialylated glycans can
be determined.
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• The MS analysis was able to detect glycan composition and sequencing information without labeling and glycosidase treatment.
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• The MS data was able to indicate the oxidized sites and linkages of glycan s.
References
1. Zhang H, Li XJ, Martin DB, Aebersold R. Nat Biotechnol. 2003, 21(6), 660-666.
2. Tian Y, Zhou Y, Elliott S, Aebersold R, Zhang H. Nat Protoc. 2007, 2(2), 334-339.
3. Van Lenten L. and Ashwell. J. G. Biol. Chem. 1971, 246, 1889–1894
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